The Science Behind HEPES Sodium Salt: Properties and Applications
HEPES Sodium Salt, identified by CAS number 75277-39-3, is a cornerstone reagent in many biological and chemical research endeavors. Its significance stems from its unique properties as a zwitterionic buffer, offering exceptional stability and utility across a broad pH spectrum. Understanding these properties is key for researchers and procurement specialists alike when considering this essential compound.
The fundamental characteristic of HEPES Sodium Salt is its robust buffering capacity, typically effective within a pH range of 6.8 to 8.2. This makes it highly suitable for biological systems that operate near neutral pH. Unlike other common buffers, HEPES Sodium Salt exhibits minimal interaction with metal ions, a property that prevents interference in assays where metal ions play a catalytic or structural role. This inertness is particularly beneficial in enzyme assays and studies involving protein interactions, where unintended chelation could lead to misleading results.
Chemically, HEPES Sodium Salt is the sodium salt of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). This salt form enhances its solubility in aqueous solutions, making it convenient for preparing buffer stocks. Its appearance as a white crystalline powder further denotes its purity, a critical factor for scientific applications. For professionals looking to buy HEPES Sodium Salt, the availability of high-purity grades, often exceeding 99.5%, from reliable manufacturers like NINGBO INNO PHARMCHEM CO.,LTD. is essential.
The applications of HEPES Sodium Salt are extensive. It is widely employed in cell culture media to maintain pH stability, especially in environments lacking precise CO2 control. Its utility extends to biochemical research, including enzyme kinetics studies, electrophoresis, and protein purification. In molecular biology, it serves as a key component in buffers for DNA and RNA isolation, helping to preserve the integrity of these vital biomolecules.
For procurement managers, sourcing HEPES Sodium Salt involves evaluating not only price but also the supplier's commitment to quality, consistency, and technical support. As a dedicated manufacturer and supplier, NINGBO INNO PHARMCHEM CO.,LTD. provides comprehensive documentation and ensures that each batch meets rigorous standards. Exploring bulk purchase options can also lead to significant cost savings for research institutions and manufacturing facilities. If you are looking for a dependable source for your HEPES Sodium Salt needs, we invite you to contact our sales team to discuss specifications and pricing.
Perspectives & Insights
Agile Reader One
“It is widely employed in cell culture media to maintain pH stability, especially in environments lacking precise CO2 control.”
Logic Vision Labs
“Its utility extends to biochemical research, including enzyme kinetics studies, electrophoresis, and protein purification.”
Molecule Origin 88
“In molecular biology, it serves as a key component in buffers for DNA and RNA isolation, helping to preserve the integrity of these vital biomolecules.”