Proteinase K is an enzyme that has earned its place as an indispensable tool in biological laboratories worldwide. Its broad enzymatic activity and resilience in challenging conditions make it ideal for a variety of applications, particularly in molecular biology. As a dedicated Proteinase K supplier, we believe in empowering researchers with a deep understanding of the products they use. Let's explore the science behind this remarkable enzyme.

At its core, Proteinase K is a serine protease, belonging to the subtilisin family. It functions by cleaving peptide bonds adjacent to the carboxyl group of aliphatic and aromatic amino acids, often those with blocked alpha-amino groups. This broad specificity is what makes it so effective at degrading a wide range of proteins, including endogenous nucleases like DNases and RNases. This ability is critical for preventing the degradation of DNA and RNA during purification procedures. When you buy Proteinase K, you are investing in a powerful tool for sample protection.

The enzyme's stability is another key characteristic. Proteinase K remains active and stable in the presence of denaturing agents such as SDS (sodium dodecyl sulfate) and urea, as well as metal chelating agents like EDTA. This is a significant advantage because these compounds are frequently used in lysis buffers to enhance cell membrane disruption and to inhibit metal-dependent nucleases. While SDS can inhibit many other enzymes, Proteinase K's activity is actually enhanced by it, as the detergent unfolds protein substrates, rendering them more accessible for cleavage. This robust performance is why reputable manufacturers, including our own operations as a Proteinase K manufacturer in China, focus on providing high-quality, stable formulations.

The primary application for Proteinase K is in the isolation of nucleic acids (DNA and RNA). By adding it to cell lysates, researchers effectively eliminate proteins that can interfere with DNA/RNA extraction or co-precipitate with the nucleic acids. This leads to cleaner samples and higher yields. Beyond nucleic acid purification, Proteinase K is also utilized in various enzyme assays, in the preparation of samples for X-ray crystallography, and in the inactivation of other enzymes.

When selecting a product, understanding the key specifications is vital. Our Proteinase K, for instance, typically has a purity of ≥ 99.9% and an activity of ≥ 30 units/mg. It is supplied as a white to off-white powder and is guaranteed to be free from detectable DNase, RNase, and nickase contamination. These specifications ensure that when you purchase Proteinase K, you are getting a reagent that will perform reliably in your sensitive molecular biology experiments.

For researchers seeking a dependable Proteinase K supplier, it is important to partner with entities that understand these scientific nuances. We are committed to providing not just a product, but a solution that enhances your research outcomes. Whether you need to buy Proteinase K online for routine lab work or specialized projects, our offering is designed to meet your stringent requirements.

In summary, the scientific basis of Proteinase K's efficacy lies in its broad specificity, stability, and ability to function effectively in the presence of common lysis agents. Its role in protecting DNA and RNA during extraction is unparalleled. We encourage you to explore our range of high-quality Proteinase K and discover the difference a reliable supplier can make to your research.