NINGBO INNO PHARMCHEM CO.,LTD. is a trusted supplier of high-quality biochemicals, and our SMCC crosslinker (CAS 64987-85-5) is a testament to our commitment to enabling scientific progress. This article highlights the critical role of SMCC in achieving precise protein-sulfhydryl and protein-amine conjugation, a fundamental technique in modern biotechnology and drug development.

SMCC, a heterobifunctional crosslinker, is designed to bridge molecules with distinct functional groups. Its N-hydroxysuccinimide (NHS) ester group exhibits high reactivity towards primary amines, commonly found on the N-terminus and lysine residues of proteins. This reaction, performed typically at pH 7.5-8.5, results in the formation of a stable amide bond, effectively tethering the SMCC to the amine-containing molecule. This is the foundation for protein-amine conjugation.

Subsequently, the maleimide moiety of SMCC reacts specifically with sulfhydryl groups, typically present in cysteine residues of proteins. This reaction, favored at pH 6.5-7.5, proceeds via Michael addition to form a stable thioether bond. This targeted reaction creates a robust linkage, ensuring the integrity of the conjugated product. This capability is central to protein-sulfhydryl conjugation.

The significance of SMCC extends across numerous scientific disciplines. In the development of Antibody-Drug Conjugates (ADCs), SMCC is often employed to link cytotoxic payloads to antibodies, creating targeted therapies with enhanced efficacy and reduced side effects. The reliable and specific conjugation facilitated by SMCC is paramount for the safety and effectiveness of these advanced pharmaceuticals. NINGBO INNO PHARMCHEM CO.,LTD. provides SMCC that meets the high standards required for such critical applications, supporting researchers in their quest for innovative treatments. We understand the importance of reliable bioconjugation reagents.

Moreover, SMCC is widely utilized for various other bioconjugation tasks, including the preparation of enzyme-antibody conjugates for diagnostic assays, the immobilization of peptides onto surfaces for biosensors, and the labeling of proteins with fluorescent tags for imaging. The efficiency and specificity of SMCC make it an indispensable tool for researchers seeking to modify and functionalize biomolecules. For those looking to leverage these capabilities, NINGBO INNO PHARMCHEM CO.,LTD. offers competitive purchase options for high-quality SMCC. The ability to perform precise protein-amine conjugation and protein-sulfhydryl conjugation makes SMCC a versatile asset.

In summary, SMCC (CAS 64987-85-5) stands out as a critical chemical linker due to its targeted reactivity with both amines and sulfhydryls. NINGBO INNO PHARMCHEM CO.,LTD. is committed to supplying the scientific community with this essential reagent, empowering advancements in biotechnology and medicine through reliable and efficient conjugation methods.