For professionals in histology and cytology, the quality of stains directly impacts the accuracy and reliability of diagnostic results. Among the most fundamental stains is Eosin Y, a vibrant dye that plays a crucial role in visualizing cellular structures. This article delves into the essential aspects of Eosin Y, from its chemical properties and applications to practical considerations for laboratory use, emphasizing the benefits of sourcing from reputable manufacturers.

Understanding Eosin Y: Properties and Chemical Identity

Eosin Y, chemically known as disodium;2-(2,4,5,7-tetrabromo-3-oxido-6-oxoxanthen-9-yl)benzoate, is a member of the xanthene dye family. Its CAS number, 17372-87-1, precisely identifies this compound. Typically appearing as a red crystalline powder, Eosin Y is highly soluble in water. Its primary function as a biological stain lies in its ability to impart a pink to red hue to the cytoplasm, intercellular substances, and collagen within tissue samples. This acidic nature makes it an ideal counterstain, commonly paired with nuclear stains like hematoxylin in the widely used Hematoxylin and Eosin (H&E) staining technique.

Key Applications in Laboratory Settings

The versatility of Eosin Y extends across several critical laboratory applications:

  • Histology and Cytology: This is arguably the most prominent use. In H&E staining, Eosin Y highlights cellular cytoplasm, muscle fibers, collagen, and red blood cells, providing essential detail for pathologists to diagnose diseases. Its role as a counterstain is indispensable for differentiating cell nuclei from their surrounding cytoplasm.
  • Adsorption Indicator: Eosin Y serves as an effective adsorption indicator in precipitation titrations, particularly for determining halide concentrations like bromide (Br-) and iodide (I-). It also finds application in the determination of other ions like silver (Ag+).
  • Spectrophotometry: In fluorescence spectrophotometry, Eosin Y is used as a color developer for quantifying various metal ions, including silver (Ag+) and lead (Pb2+).

Ensuring Quality: Sourcing Eosin Y from a Reliable Supplier

For any laboratory, consistency and purity of reagents are paramount. When you buy Eosin Y, it's crucial to source it from a reputable manufacturer in China or a trusted distributor. A certified biological stain guarantees that the product meets stringent quality standards, ensuring reproducible results and accurate diagnoses. Factors like maximum absorption wavelength and mass absorption coefficient are critical specifications that a good supplier will clearly provide. For those looking to purchase Eosin Y for their histology labs or research projects, partnering with a reliable supplier that offers competitive Eosin Y stain price is essential. This ensures not only cost-effectiveness but also the integrity of your experimental outcomes.

Practical Considerations for Eosin Y Usage

When using Eosin Y, understanding its properties is key. As an acidic dye, it stains basic (eosinophilic) components of cells. It is often used in an aqueous solution, but can also be formulated in alcohol. Proper storage, typically in a cool, dry place protected from light, will maintain its efficacy. Laboratories often prepare their own staining solutions or purchase ready-to-use formulations. Regardless of the method, maintaining the correct pH and concentration is vital for optimal staining. If you are seeking to buy Eosin Y Disodium Salt with CAS 17372-87-1, look for suppliers who emphasize their certification and quality control processes. The right Eosin Y supplier will support your laboratory’s needs for high-quality diagnostic reagents.

In conclusion, Eosin Y is a cornerstone stain in biological sciences, offering unparalleled utility in both diagnostic pathology and analytical chemistry. By understanding its applications and prioritizing quality sourcing from experienced manufacturers, laboratories can ensure the highest standards of precision and reliability in their work.