The Essential Role of Bicine Buffers in Modern Biochemical Research
In the dynamic field of biochemical research, maintaining precise and stable pH conditions is not just a requirement but the cornerstone of reliable experimental outcomes. Among the plethora of buffering agents available, N,N-Bis(2-hydroxyethyl)glycine, commonly known as Bicine, has carved a significant niche. This article delves into why Bicine is such a favored compound in laboratories worldwide and how researchers can efficiently source it for their critical experiments.
Bicine, with its CAS number 150-25-4, is an amphoteric molecule. This means it possesses both acidic and basic functional groups – specifically, a carboxylic acid group and a tertiary amine group. This dual nature allows Bicine to effectively resist changes in pH when small amounts of acid or base are added. Its buffering range, typically between pH 7.6 and 9.0, makes it particularly suitable for a variety of biological systems that operate within or near physiological pH. Many enzymatic reactions, protein studies, and cell culture applications require such a stable environment, and Bicine consistently delivers.
The advantages of using Bicine extend beyond its buffering capacity. It exhibits good solubility in water, which simplifies the preparation of stock solutions. Furthermore, compared to some other common biological buffers, Bicine is often found to have lower metal ion binding affinity, which can be critical when working with metal-sensitive enzymes or assays. Understanding the specific 2-(Bis(2-hydroxyethyl)amino)acetic acid properties is key for researchers to optimize their experimental designs. For scientists looking to purchase Bicine, ensuring high purity is paramount to avoid introducing unwanted contaminants that could interfere with sensitive experiments. NINGBO INNO PHARMCHEM CO.,LTD. is a reliable source for high-quality Bicine, enabling researchers to achieve accurate and reproducible results. The ability to buy Bicine for your lab from a trusted supplier like NINGBO INNO PHARMCHEM CO.,LTD. streamlines the procurement process, allowing scientists to focus more on their research rather than on sourcing materials.
The applications of Bicine are diverse. It is frequently employed in electrophoresis buffers, enzyme kinetics studies, and as a component in cell culture media. Its stability and effectiveness in the physiological pH range are highly valued. The compound's role is not limited to the lab; its chemical properties also lend themselves to industrial applications such as gas sweetening, where pH control is crucial. When considering the buy 2-(Bis(2-hydroxyethyl)amino)acetic acid options, it's wise to consult with suppliers like NINGBO INNO PHARMCHEM CO.,LTD. who can provide detailed specifications and certifications to meet stringent research requirements. Effectively utilizing 2-(Bis(2-hydroxyethyl)amino)acetic acid in your biochemical protocols can significantly enhance the reliability and validity of your findings.
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