In the dynamic field of protein science and biotechnology, the ability to precisely control the state of protein molecules is paramount. DL-Dithiothreitol (DTT), often referred to by its discoverer's name, Cleland's Reagent, stands as a cornerstone chemical for researchers and manufacturers alike. Its primary function as a potent reducing agent makes it indispensable for a variety of critical laboratory and industrial processes. Understanding the capabilities and sourcing DTT effectively can significantly impact experimental success and product quality.

What is DL-Dithiothreitol (DTT)?
DTT is an organosulfur compound characterized by its two thiol groups. These groups are highly reactive and are responsible for DTT's remarkable ability to reduce disulfide bonds (-S-S-) back to their sulfhydryl (-SH) forms. This reduction is fundamental in preventing or reversing the formation of unwanted disulfide cross-links in proteins. These cross-links can lead to protein misfolding, aggregation, and loss of biological activity, issues that researchers strive to avoid.

Key Applications in Biotechnology and Research
The versatility of DTT is evident in its widespread use across several scientific disciplines. In protein biochemistry, DTT is routinely added to buffers during protein purification and analysis. It ensures that cysteine residues remain in their reduced state, preventing premature oxidation that could lead to aberrant disulfide bond formation. This is particularly crucial for techniques like SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), where DTT is often included in sample preparation to fully denature proteins by reducing internal disulfide bonds, allowing for accurate separation based on molecular weight.

Beyond protein work, DTT plays a role in molecular biology, aiding in the protection of sensitive thiol groups in enzymes and nucleic acids. Its application extends to protecting thiolated DNA from dimerization, which is vital for subsequent coupling reactions such as in biosensor development.

For product development and manufacturing, sourcing high-quality DTT is essential. As a reliable manufacturer in China, we understand the stringent purity requirements of the pharmaceutical and biotechnology industries. Our commitment to quality ensures that the DTT you purchase meets the highest standards, guaranteeing consistent performance in your sensitive applications. Knowing the correct DTT price is also a critical factor for procurement managers looking for cost-effective solutions without compromising on quality.

Handling and Storage Considerations
While invaluable, DTT requires careful handling and storage. It is sensitive to air oxidation, meaning its reducing power can be compromised over time, especially when exposed to oxygen. Therefore, it is typically stored desiccated at 2-8°C under an inert atmosphere (like argon or nitrogen) to maintain its stability and efficacy for up to three years when properly stored. For immediate use, preparing fresh DTT solutions is recommended, as solutions can degrade relatively quickly, particularly at warmer temperatures or higher pH levels.

Procuring DTT Effectively
When seeking to buy DTT, partnering with a reputable supplier who prioritizes product integrity is key. We offer a consistent supply of high-purity DL-Dithiothreitol, catering to both research quantities and bulk industrial needs. Our team is dedicated to providing excellent customer service, including competitive pricing and timely delivery, ensuring your research and manufacturing processes remain uninterrupted. If you are looking for a dependable DTT manufacturer, we invite you to contact us for inquiries and quotes.

In conclusion, DL-Dithiothreitol is an indispensable reagent for anyone working with proteins or nucleic acids. Its ability to control redox states makes it a powerful tool in laboratories worldwide. By understanding its applications, properties, and proper handling, and by sourcing from reliable manufacturers, you can effectively leverage DTT's capabilities for your scientific and commercial endeavors.