6-Carboxyfluorescein (CAS 3301-79-9) is a fascinating molecule that bridges the gap between chemistry and biology, empowering researchers with its remarkable fluorescent capabilities. As a derivative of fluorescein, it inherits the parent molecule's vibrant fluorescence while gaining a crucial functional group – a carboxyl moiety. This seemingly small addition significantly expands its utility in scientific applications, making it a sought-after product for anyone looking to buy fluorescent dyes.

Chemically, 6-Carboxyfluorescein possesses a complex structure characterized by its xanthene core, fused aromatic rings, and two hydroxyl groups, along with the critical carboxyl group. This structure dictates its photophysical properties. When exposed to excitation light, typically around 492-495 nm, it efficiently absorbs energy and re-emits it as visible light, usually around 517-518 nm. This strong fluorescence makes it an excellent reporter molecule. The purity of the synthesized compound, often specified as ≥97%, is paramount to ensure optimal spectral performance and prevent interference from impurities that could quench fluorescence or shift emission wavelengths.

The versatility of 6-Carboxyfluorescein arises from its carboxyl group. This functional handle can be readily activated and conjugated to biomolecules that possess primary amine groups, such as lysine residues on proteins or amines on modified nucleotides. This process, often facilitated by carbodiimide chemistry, allows scientists to covalently attach the fluorescent tag to their molecules of interest. This capability is fundamental for various applications:

  • Nucleic Acid Synthesis and Analysis: In the synthesis of DNA and RNA strands for sequencing, PCR, and microarray technologies, 6-Carboxyfluorescein is incorporated to create fluorescently labeled probes. This allows for real-time detection and quantification of genetic material. Buyers often search for 6-carboxyfluorescein manufacturer offerings for these specific needs.
  • Bioconjugation and Labeling: For protein and peptide labeling, the dye provides a sensitive tag for detection in assays like Western blotting or fluorescence microscopy. It's also used in creating FRET (Förster Resonance Energy Transfer) pairs.
  • Cellular and In Vivo Tracing: When introduced into cells or organisms, its fluorescence can track cellular processes, monitor drug delivery systems like liposomes, or study fluid dynamics within living systems.

The demand for high-quality 6-Carboxyfluorescein fuels a significant market for its suppliers. When procuring this compound, understanding its specifications and the reputation of the fluorescent dye supplier is essential. Buyers are often interested in the CAS 3301-79-9 price, but equally important are factors like batch consistency, documentation (COA, TDS), and shipping reliability. Partnering with a manufacturer that specializes in fluorescent organic compounds ensures access to compounds that meet rigorous scientific standards.

In summary, the scientific elegance of 6-Carboxyfluorescein lies in its chemical design, which bestows powerful fluorescent properties upon it. Its ability to be conjugated to diverse biological entities opens doors to advanced research methodologies. For any scientist or manufacturer requiring reliable fluorescent labeling capabilities, understanding the science behind this compound and sourcing it from a trusted provider is a crucial first step.