The intricate world of molecular biology often relies on sophisticated tools for labeling and tracking biomolecules. Biotinylation reagents are among the most versatile, and Biotin-AC5-AC5-OSU (Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester, CAS 89889-52-1) is a prime example of a reagent meticulously designed for such purposes. NINGBO INNO PHARMCHEM CO.,LTD., a leading chemical synthesis company, provides this high-purity compound, allowing researchers to delve deeper into its scientific applications. This article examines the structure and function of Biotin-AC5-AC5-OSU, highlighting its importance in various biological research contexts.

The chemical architecture of Biotin-AC5-AC5-OSU is key to its functionality. It comprises three main components: the biotin moiety, a flexible spacer arm, and an activated NHS ester. The biotin molecule is well-known for its exceptionally high affinity for avidin and streptavidin, forming one of the strongest non-covalent biological interactions known. This strong binding is the basis for many detection and purification assays. The spacer arm in Biotin-AC5-AC5-OSU consists of two aminohexanoic acid units, providing a significant length (approximately 30.5 Å). This extended linker is crucial as it reduces steric hindrance between the biotin tag and the molecule it is attached to, as well as between the biotin and its binding partner (avidin/streptavidin). This design ensures that the biotinylated molecule remains accessible and functional, a critical consideration when researchers choose to buy reagents for sensitive applications.

The N-hydroxysuccinimide (NHS) ester is the reactive group that facilitates the covalent attachment of the biotin-spacer complex to target molecules. NHS esters are widely used in bioconjugation chemistry because they are highly reactive towards primary amines under physiological pH conditions (typically pH 7-9). The reaction proceeds via nucleophilic acyl substitution, forming a stable amide bond. This specificity for amines, commonly found on lysine residues and N-terminal amino groups of proteins, makes Biotin-AC5-AC5-OSU a targeted labeling agent. The reliability of this reaction is enhanced by the high purity (>95%) of the reagent, ensuring consistent conjugation efficiency, a hallmark of quality provided by manufacturers like NINGBO INNO PHARMCHEM CO.,LTD.

The scientific applications stemming from this structure-function relationship are vast. In protein modification, Biotin-AC5-AC5-OSU is used to create biotinylated antibodies for Western blotting, ELISA, and immunohistochemistry, enabling sensitive detection. Researchers also employ it in affinity chromatography to purify proteins that bind to biotinylated ligands. Furthermore, in cell biology, it is utilized for labeling cell surface proteins or intracellular components, facilitating studies in cell imaging, flow cytometry, and receptor-ligand binding assays. The consistent performance of this reagent, when purchased from a trusted supplier such as NINGBO INNO PHARMCHEM CO.,LTD., allows for reproducible experimental results, which is fundamental for scientific validity.

The chemical synthesis expertise at NINGBO INNO PHARMCHEM CO.,LTD. ensures that Biotin-AC5-AC5-OSU is produced to meet the stringent demands of scientific research. Understanding the underlying science of such reagents empowers researchers to select the most appropriate tools for their specific experiments. The robust nature of the biotin-avidin interaction, combined with the targeted reactivity of the NHS ester and the advantageous spacer arm of Biotin-AC5-AC5-OSU, makes it an indispensable reagent in the modern biological toolkit.

In summary, the intricate design of Biotin-AC5-AC5-OSU, combining the strong binding of biotin with a reactive NHS ester via an extended spacer, underpins its utility in diverse biological applications. NINGBO INNO PHARMCHEM CO.,LTD. plays a crucial role in enabling scientific progress by manufacturing and supplying this high-quality reagent, allowing researchers to confidently buy and utilize it for accurate and efficient molecular labeling.