In the field of life sciences, the precision of experimental conditions often dictates the success of research outcomes. Buffers, in particular, play an indispensable role by maintaining stable pH levels, crucial for the integrity and function of biological molecules. Among the prominent buffers, HEPPS (4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid), identified by CAS number 16052-06-5, has emerged as a highly regarded choice for scientists and researchers. As a dedicated manufacturer and supplier, we aim to illuminate the scientific underpinnings of this versatile compound.

HEPPS belongs to the family of Good's buffers, characterized by their zwitterionic nature and suitability for biological research. Its chemical structure, featuring a piperazine ring functionalized with both a hydroxyethyl group and a propanesulfonic acid tail, imparts a unique set of properties. These include high water solubility, a stable buffering capacity in the physiological pH range (approximately pH 7.3-8.7), and a pKa value close to 8.0 at 25°C. This makes it an ideal buffer for applications requiring a pH slightly above neutral.

The utility of HEPPS spans numerous scientific disciplines. In cell biology, it is integral to the formulation of cell culture media, supporting optimal cellular health and function by preventing detrimental pH fluctuations. Researchers frequently use HEPPS to enhance protein delivery and transfection efficiency in mammalian cells, contributing to advancements in genetic engineering and disease modeling. For those seeking to purchase HEPPS for cell culture, understanding these specific benefits can inform their procurement strategy.

Biochemical research heavily relies on HEPPS for various applications, including enzyme kinetics studies, protein purification, and protein crystallization. The buffer's stability and low metal-chelating properties ensure that it does not interfere with enzymatic activity or protein interactions. Its minimal UV absorbance also makes it suitable for spectrophotometric monitoring of reactions. When you buy HEPPS, you are acquiring a tool that enhances the reliability and reproducibility of biochemical experiments.

Moreover, HEPPS has found its way into molecular biology protocols. It can be incorporated into buffer systems for DNA and RNA isolation, ensuring the stability of nucleic acids during extraction and manipulation. Its compatibility with PCR and other amplification techniques further underscores its versatility. Laboratories requiring a consistent supply of high-purity HEPPS can look to reliable manufacturers to meet their needs.

The manufacturing process for HEPPS is crucial to its quality. As a producer, we employ stringent quality control measures to ensure an assay of at least 99%. This commitment to purity is vital, as impurities can lead to artifactual results or inhibit biological processes. Our facility in China is equipped to produce HEPPS at scale, offering competitive pricing and ensuring a steady supply for both research and industrial demands. We encourage prospective buyers to inquire about our product specifications and obtain a quote.

In summary, HEPPS is more than just a buffer; it is a foundational reagent that enables critical research in biology and chemistry. Its well-defined properties and broad applicability make it an essential component in any well-equipped laboratory. We are proud to be a trusted manufacturer and supplier, facilitating scientific progress through the provision of high-quality HEPPS.