In the realm of chemical reagents, understanding the fundamental properties of a compound is key to its effective application. MES Sodium Salt (CAS 71119-23-8) is a prime example of a chemical that, while seemingly simple, possesses a sophisticated profile of attributes making it indispensable in various scientific and industrial fields. As a prominent manufacturer and supplier of MES Sodium Salt from China, we are pleased to share insights into the science behind this critical buffering agent.

MES Sodium Salt is chemically known as 2-(N-Morpholino)ethanesulfonic acid sodium salt. Its molecular formula is C6H12NNaO4S, and it has a molecular weight of 217.22 g/mol. The defining characteristic of MES Sodium Salt, like other zwitterionic compounds, is its ability to carry both positive and negative charges within the same molecule, contributing to its high water solubility and unique buffering behavior. It is classified as one of the 'Good's buffers,' a series of buffers developed for biological research based on stringent criteria, including a useful pKa, high water solubility, and minimal biological interaction.

The pKa value of MES Sodium Salt is approximately 6.1 at 20°C. This means that the buffer is most effective at maintaining a stable pH close to this value. The optimal buffering range for MES Sodium Salt is generally considered to be between pH 5.5 and 6.5. This pH range is critical for many biological systems, including enzyme activity, protein stability, and cell culture environments. For researchers and formulators, understanding this pKa is the first step in determining if MES Sodium Salt is the right choice for their specific needs. When you are looking to buy MES Sodium Salt, knowing this property is crucial for its effective application.

Beyond its buffering capabilities, MES Sodium Salt offers several other significant advantages:

  • Low UV Absorbance: In applications requiring spectrophotometric analysis, such as enzyme kinetics or protein concentration determination, minimal interference from the buffer is essential. MES Sodium Salt exhibits very low absorbance in the UV range (typically < 0.1 at 280 nm for a 10% solution), making it an excellent choice for such sensitive measurements.
  • Minimal Metal Ion Binding: Unlike some other common buffers, MES Sodium Salt has a low tendency to complex with metal ions. It forms weak complexes with Ca2+, Mg2+, and Mn2+, and essentially no complex with Cu2+. This property is invaluable in biological and biochemical experiments where the concentration and activity of free metal ions need to be precisely controlled.
  • Chemical Stability: MES Sodium Salt is chemically stable under typical laboratory conditions and is resistant to enzymatic degradation. This stability ensures consistent buffering performance over time and across different experimental setups.

The practical applications of MES Sodium Salt are a testament to its beneficial properties:

  • Biochemical and Biological Research: It is widely used in fundamental research for maintaining pH in enzyme assays, protein purification, and cellular studies.
  • Cell Culture Media: Providing a stable pH is vital for cell viability and growth. MES Sodium Salt is frequently incorporated into cell culture media formulations.
  • Electrophoresis: As a component of electrophoresis buffers, it helps maintain a stable pH during the separation of biomolecules, ensuring reliable results.
  • Chromatography: Its buffering capacity and low ionic strength contribution make it suitable for various chromatographic techniques, including ion-exchange and affinity chromatography.
  • Diagnostic Kits: In the development and manufacturing of diagnostic reagents, the purity and buffering stability of MES Sodium Salt are critical for assay performance and product shelf-life.

As a leading MES Sodium Salt manufacturer and supplier, we ensure that our product meets high standards of purity and quality. We understand that reliable sourcing is key for our clients, and we are committed to providing competitive MES Sodium Salt price points and consistent supply. If you require this essential buffering agent for your research or industrial processes, we encourage you to contact us for a quotation and to discuss your specific needs. Partnering with a trusted supplier ensures you receive the quality and reliability you depend on.