The precision required in biochemical analysis often hinges on the quality of the reagents used. For scientists focusing on peptide and low molecular weight protein separation, Tricine (CAS 5704-04-1) has emerged as a critical component. As a reputable manufacturer and supplier of fine chemicals, we understand the unique demands of laboratories performing advanced separation techniques like SDS-PAGE.

Tricine is specifically lauded for its role in modified SDS-PAGE systems, often referred to as Tricine-SDS-PAGE. Unlike standard Tris-Glycine buffers, Tricine-SDS-PAGE is optimized for resolving smaller peptides and proteins, typically those falling below 10 kDa, though its effectiveness can extend up to 100 kDa. The key advantage lies in its buffering mechanism; Tricine functions as a trailing ion, meaning it moves slower than the sample components and the leading ion, which helps to establish a stable ion front. This characteristic is crucial for achieving sharp bands and high resolution, enabling researchers to accurately identify and quantify their target molecules. If your research involves detailed proteomic analysis or peptide mapping, purchasing high-purity Tricine from a trusted chemical supplier is a prudent step.

The effectiveness of Tricine in these demanding applications stems from its chemical properties. Its pKa value of approximately 8.1 makes it an excellent buffer in the slightly alkaline range, providing the stable pH environment necessary for the integrity of peptides and proteins during electrophoresis. Furthermore, its relatively low ionic mobility compared to Tris or Glycine contributes to the distinct separation patterns achieved in Tricine-SDS-PAGE. This specificity makes it an indispensable tool for many molecular biology and biochemistry laboratories.

Beyond its primary use in electrophoresis, the applications of Tricine are noteworthy. It is employed in various biochemical assays and as a component in cell culture media where pH stability is critical. Its role as a biological buffer also extends to its use in cosmetics and detergents due to its stability and mildness. For procurement managers looking to buy Tricine in bulk for industrial or research purposes, exploring options from reliable chemical manufacturers in China can offer significant cost benefits without compromising on quality.

When sourcing Tricine, it is important to consider the purity and specifications. A minimum assay of 99% is generally recommended for sensitive applications like electrophoresis to ensure the absence of interfering impurities. As a leading supplier, we provide detailed technical specifications and ensure our products meet stringent quality control standards. We encourage researchers and purchasing agents to request quotes and samples to verify the quality and suitability of our Tricine for their specific workflows. Investing in high-quality reagents is an investment in the accuracy and reliability of your scientific outcomes.