In the critical process of protein separation via electrophoresis, the choice of buffer is paramount. While glycine has been a long-standing staple, Tricine, identified by CAS 5704-04-1, offers distinct advantages, particularly for the resolution of low molecular weight proteins and peptides. This article delves into the comparison between Tricine and glycine as buffer components, highlighting why Tricine is often the preferred choice for advanced applications in molecular biology and biochemistry.

Glycine-based buffers are widely used in standard SDS-PAGE. However, they can exhibit limitations when resolving proteins below 20 kDa, often resulting in diffused bands and reduced accuracy. Tricine, on the other hand, is specifically designed to overcome these challenges. Its chemical structure, N-[Tris(hydroxymethyl)methyl]glycine, allows it to function effectively as a trailing ion in SDS-PAGE, leading to significantly sharper band resolution. This enhanced separation capability is a key reason behind the widespread adoption of Tricine-SDS-PAGE systems in research labs seeking precise analysis of smaller proteins and peptides.

The difference in performance stems from the ionic properties of Tricine. At typical running pH values (7.4-8.8), Tricine carries a greater negative charge compared to glycine. This influences its migration through the gel matrix and its interaction with the SDS-protein complexes, ultimately contributing to the superior resolution observed. The predictable behavior of Tricine makes tricine buffer preparation straightforward, ensuring consistent experimental outcomes. Researchers often ask what Tricine is used for in biochemistry, and its role in improving electrophoretic resolution is a primary answer.

Furthermore, the availability of high purity Tricine for research is essential for achieving reproducible results. When considering to buy Tricine, it is crucial to source from reliable suppliers. NINGBO INNO PHARMCHEM CO.,LTD. provides Purity Degree 99% Tricine CAS No. 5704-04-1, ensuring that scientists have access to a premium-grade reagent that supports their most demanding analytical needs. The choice to use Tricine over glycine is a strategic one for researchers prioritizing accuracy and detail in protein analysis.