Understanding Agarose Specifications for Different Lab Needs
For professionals in the chemical and life science industries, selecting the appropriate grade of Agarose is crucial for the success of various experimental procedures. Agarose, a polysaccharide with CAS 9012-36-6, is available in different specifications tailored for diverse applications, ranging from basic molecular biology to advanced biochemical assays. NINGBO INNO PHARMCHEM CO.,LTD., a leading manufacturer and supplier, provides insights into these critical specifications, helping researchers and procurement managers make informed purchasing decisions.
One of the most important specifications for Agarose used in nucleic acid electrophoresis is its Electro-endosmosis (EEO) value. EEO refers to the movement of buffer ions and the resulting electroosmotic flow within the gel matrix. High EEO can impede the migration of DNA and RNA, leading to distorted bands and reduced resolution. Therefore, for sensitive applications like DNA fragment analysis, PCR, and Southern blotting, Agarose with a low EEO value (typically < 0.15 mr) is highly recommended. When you decide to buy Agarose, checking this specification is vital.
Gel strength is another critical parameter. It quantifies the ability of the Agarose gel to withstand mechanical stress. A higher gel strength ensures that the gel remains stable during handling and electrophoresis, preventing it from breaking or deforming. For gels requiring higher concentrations of Agarose or for applications demanding very firm matrices, selecting an Agarose with a higher gel strength (e.g., 1200 g/cm² or more) is beneficial. As a reliable Agarose supplier, we offer products with robust gel strengths suitable for demanding applications.
Purity is a universal requirement, and for Agarose, it translates to the absence of contaminants that could interfere with experimental outcomes. High-purity Agarose (often 99% or greater) is essential for applications where even trace impurities could inhibit enzymes, affect DNA binding, or contribute to background signal. Laboratories should always seek to buy Agarose that has been certified for purity and tested for nucleases. Our manufacturing processes ensure that you receive Agarose of the highest quality.
The melting and gelling temperatures also play a role, especially in applications involving sensitive biological molecules or when preparing gels for specific temperatures. Low-melting point Agarose is useful for applications where the sample might be damaged at higher temperatures or needs to be recovered from the gel. NINGBO INNO PHARMCHEM CO.,LTD. offers a range of Agarose products to meet these varied requirements. We encourage you to contact us for a quote and to discuss your specific needs for Agarose, ensuring you get the right product from a trusted supplier.
Perspectives & Insights
Quantum Pioneer 24
“The melting and gelling temperatures also play a role, especially in applications involving sensitive biological molecules or when preparing gels for specific temperatures.”
Bio Explorer X
“Low-melting point Agarose is useful for applications where the sample might be damaged at higher temperatures or needs to be recovered from the gel.”
Nano Catalyst AI
“We encourage you to contact us for a quote and to discuss your specific needs for Agarose, ensuring you get the right product from a trusted supplier.”