In the complex landscape of chemical manufacturing and research, specific reagents become indispensable due to their unique reactivity and versatility. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, widely recognized by its acronym EDC HCl (or EDCI), is one such reagent. Primarily functioning as a water-soluble carbodiimide, EDC HCl is a cornerstone in various synthetic pathways, most notably in peptide synthesis and bioconjugation. Understanding its properties, applications, and safe handling practices is crucial for any professional in the chemical or pharmaceutical industries.

Chemical Profile of EDC HCl

EDC HCl, identified by CAS number 25952-53-8, typically presents as a white to pale yellow crystalline powder. Its molecular formula is C8H18ClN3, and it possesses a molecular weight of approximately 191.70 g/mol. The defining characteristic of EDC HCl is its remarkable water solubility, which differentiates it from many other carbodiimide coupling agents. This solubility extends to its urea byproduct, a feature that significantly simplifies reaction workups and purification processes. Assay values commonly range around 99.0% or higher, underscoring its suitability for demanding applications where purity is critical.

Key Applications and Mechanisms

The primary role of EDC HCl is as a coupling reagent. It excels at activating carboxyl groups, transforming them into reactive intermediates that readily form amide bonds with primary amines. This mechanism is fundamental to the synthesis of peptides, where amino acids are sequentially linked. Beyond peptide synthesis, EDC HCl is a vital tool for bioconjugation, enabling the covalent attachment of biomolecules such as proteins and nucleic acids to surfaces or labels. It acts as a dehydrant, facilitating condensation reactions by removing water. For those looking to buy EDC HCl, its broad applicability makes it a valuable addition to any chemical toolkit.

Safety and Handling Considerations

While EDC HCl is a highly effective reagent, it is essential to handle it with appropriate care. It is generally considered a skin sensitizer and can cause irritation. Therefore, wearing suitable personal protective equipment (PPE), including gloves, eye protection, and protective clothing, is paramount. Reactions should ideally be conducted in well-ventilated areas. Proper storage, typically in a cool, dry place, away from moisture, is also recommended to maintain its efficacy. Adhering to safety data sheets and best practices ensures the safe and effective use of EDC HCl.

Sourcing EDC HCl from a Reliable Manufacturer

For businesses and researchers requiring a consistent supply of high-quality EDC HCl, partnering with a trusted manufacturer is essential. NINGBO INNO PHARMCHEM CO.,LTD. is a premier EDC HCl supplier, committed to providing pure and reliable reagents. We offer competitive pricing, especially for bulk purchases, and ensure prompt delivery. If you are seeking to buy EDC HCl for your peptide synthesis, bioconjugation, or other chemical manufacturing needs, our team is equipped to provide the support and products you require. Contact us to discuss your specific requirements and discover the difference quality sourcing makes.