Fmoc-Hyp(tBu)-OH, chemically known as Fmoc-4-tert-butoxy-L-proline, is a vital reagent in contemporary organic synthesis, particularly within the realm of peptide chemistry. Its unique structural features and the reliability it offers in synthesis protocols make it a preferred choice for researchers.

The compound's identity is firmly established by its CAS number, 122996-47-8, which serves as a universal reference for its chemical specifications. Its molecular formula, C24H27NO5, and molecular weight of approximately 409.47 g/mol, provide essential details for chemists. The defining characteristic of Fmoc-Hyp(tBu)-OH is the presence of the Fmoc (9-fluorenylmethoxycarbonyl) protecting group on the amine functionality and a tert-butyl ether protecting group on the hydroxyl of the hydroxyproline ring. This dual protection strategy is fundamental to its utility in solid-phase peptide synthesis (SPPS). When researchers choose to buy Fmoc-Hyp(tBu)-OH, they are investing in a reagent that ensures selective deprotection and efficient coupling, leading to higher yields and purer peptide products. Many suppliers, including those based in China, offer this compound, allowing for competitive pricing and accessibility.

The synthesis of Fmoc-Hyp(tBu)-OH typically involves the selective protection of L-hydroxyproline. The tert-butyl protection of the hydroxyl group is crucial because it is stable under the basic conditions used for Fmoc deprotection but can be removed under acidic conditions, often during the final cleavage of the peptide from the resin. This orthogonality in protecting groups is a key principle in efficient peptide synthesis. Understanding these chemical properties is vital for optimizing synthesis protocols. The availability of this reagent from companies like NINGBO INNO PHARMCHEM CO.,LTD. ensures that academic and industrial laboratories have access to the materials needed for cutting-edge research.

The applications of Fmoc-Hyp(tBu)-OH extend to the creation of peptides with modified structures, which can possess enhanced stability against enzymatic degradation or exhibit unique conformational properties. This makes it invaluable in fields ranging from medicinal chemistry and drug discovery to the development of novel biomaterials. The consistent quality and availability of this compound are therefore critical factors for the successful progression of these research endeavors.