Understanding N-Cyclohexyltaurine: Properties for Advanced Scientific Applications
For researchers dedicated to advancing scientific frontiers, understanding the fundamental properties of the reagents they use is paramount. N-Cyclohexyltaurine, often referred to by its acronym CHES, is a chemical compound that has garnered significant attention in the scientific community due to its utility as a biological buffer. Its unique chemical makeup and resulting properties make it an essential component in a wide array of advanced scientific applications, particularly in enzymology and molecular biology.
The chemical identity of N-Cyclohexyltaurine is defined by its molecular formula, C8H17NO3S, and a molecular weight of 207.29 g/mol. This structure imparts zwitterionic characteristics, which are key to its buffering capabilities. The pKa value of CHES is approximately 9.3 at 25°C, indicating that it is most effective at buffering in the alkaline pH range, specifically between 8.6 and 10.0. This makes it an ideal choice for experiments that require a stable pH in this specific range, where other common buffers might be less effective or even interfere with the reaction.
The physical properties of N-Cyclohexyltaurine are also noteworthy. It typically presents as a white crystalline powder, a form that is convenient for laboratory use and storage. Its solubility in water is good, with a capacity of up to 0.5 M at 20°C, yielding a clear, colorless solution. This solubility is critical for preparing buffer solutions of desired concentrations, facilitating precise experimental setups.
In terms of stability, N-Cyclohexyltaurine is generally considered stable under standard storage conditions. However, it is noted to be hygroscopic, meaning it can absorb moisture from the air, underscoring the importance of proper storage in sealed containers. Its low reactivity with most metal ions is another significant advantage, preventing unintended complexation or catalytic activity that could skew experimental results.
These properties collectively make CHES an excellent buffer for protein stabilization and a wide range of enzyme research applications. Researchers looking to purchase CHES can find it from various chemical suppliers, ensuring access to this vital reagent for their demanding scientific endeavors. By understanding and leveraging these fundamental properties, scientists can optimize their experimental designs and achieve more accurate and reliable outcomes.
The chemical identity of N-Cyclohexyltaurine is defined by its molecular formula, C8H17NO3S, and a molecular weight of 207.29 g/mol. This structure imparts zwitterionic characteristics, which are key to its buffering capabilities. The pKa value of CHES is approximately 9.3 at 25°C, indicating that it is most effective at buffering in the alkaline pH range, specifically between 8.6 and 10.0. This makes it an ideal choice for experiments that require a stable pH in this specific range, where other common buffers might be less effective or even interfere with the reaction.
The physical properties of N-Cyclohexyltaurine are also noteworthy. It typically presents as a white crystalline powder, a form that is convenient for laboratory use and storage. Its solubility in water is good, with a capacity of up to 0.5 M at 20°C, yielding a clear, colorless solution. This solubility is critical for preparing buffer solutions of desired concentrations, facilitating precise experimental setups.
In terms of stability, N-Cyclohexyltaurine is generally considered stable under standard storage conditions. However, it is noted to be hygroscopic, meaning it can absorb moisture from the air, underscoring the importance of proper storage in sealed containers. Its low reactivity with most metal ions is another significant advantage, preventing unintended complexation or catalytic activity that could skew experimental results.
These properties collectively make CHES an excellent buffer for protein stabilization and a wide range of enzyme research applications. Researchers looking to purchase CHES can find it from various chemical suppliers, ensuring access to this vital reagent for their demanding scientific endeavors. By understanding and leveraging these fundamental properties, scientists can optimize their experimental designs and achieve more accurate and reliable outcomes.
Perspectives & Insights
Nano Explorer 01
“3 at 25°C, indicating that it is most effective at buffering in the alkaline pH range, specifically between 8.”
Data Catalyst One
“This makes it an ideal choice for experiments that require a stable pH in this specific range, where other common buffers might be less effective or even interfere with the reaction.”
Chem Thinker Labs
“It typically presents as a white crystalline powder, a form that is convenient for laboratory use and storage.”