The ABTS assay, utilizing ABTS ammonium salt (CAS 30931-67-0), has become a cornerstone in various analytical fields, particularly in biochemistry and food science. As a premier manufacturer and supplier, we aim to equip researchers and procurement managers with a clear understanding of this versatile reagent. This article delves into the core aspects of the ABTS assay, its importance, and what to consider when you need to buy ABTS ammonium salt.

At its heart, the ABTS assay is a colorimetric method that relies on the enzymatic oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by peroxidase enzymes, such as Horseradish Peroxidase (HRP), in the presence of hydrogen peroxide. The result is the formation of a stable, blue-green colored radical cation (ABTS•+), which can be measured spectrophotometrically. This characteristic color change is the basis for its widespread use in detecting enzyme activity and quantifying antioxidant capacities.

Key Applications of ABTS Ammonium Salt

One of the most prominent applications is in Enzyme-Linked Immunosorbent Assays (ELISA). In ELISA, an enzyme conjugate (often HRP) is used, and ABTS ammonium salt serves as the substrate. When the enzyme acts on ABTS, it produces a quantifiable color signal that corresponds to the amount of analyte present in the sample. For laboratories performing diagnostic tests or immunoassay research, sourcing high-purity ABTS ammonium salt from a reliable supplier is crucial for assay sensitivity and reproducibility.

Beyond immunoassays, the ABTS assay is extensively used to determine the total antioxidant capacity (TAC) of various substances, including plant extracts, foods, and biological fluids. This is often referred to as the ABTS/TEAC (Trolox Equivalent Antioxidant Capacity) assay. Researchers interested in the health benefits of foods or the efficacy of potential antioxidant compounds frequently need to buy ABTS ammonium salt for these studies. The ability of an antioxidant to quench the ABTS radical cation leads to a decrease in the color intensity, allowing for its measurement.

What to Consider When Buying ABTS Ammonium Salt

When you are looking to buy ABTS ammonium salt, several factors are paramount. Firstly, purity is key. For most applications, especially sensitive biochemical assays, a purity of ≥99.0% is essential. As a leading manufacturer, we ensure our ABTS ammonium salt meets these stringent quality standards. Secondly, consider the form and solubility. ABTS ammonium salt is typically supplied as a light green powder and is soluble in water, which simplifies its preparation and use in aqueous assay systems.

Sourcing from a reliable manufacturer and supplier like NINGBO INNO PHARMCHEM CO.,LTD. is vital. We offer competitive price inquiries and ensure consistent supply chain reliability. Whether you need small quantities for laboratory research or bulk orders for industrial applications, understanding the supplier's capacity and quality control measures is important. If you are looking to purchase ABTS ammonium salt in China, working with established chemical manufacturers provides assurance of product quality and efficient logistics.

In conclusion, the ABTS assay, powered by high-quality ABTS ammonium salt, is an indispensable tool in modern analytical chemistry. Whether your focus is on immunoassay development, food analysis, or antioxidant research, investing in a reputable supplier for your ABTS ammonium salt needs will directly impact the success and reliability of your scientific endeavors.