In the intricate world of chemical synthesis and biological research, a deep understanding of a compound's properties is key to unlocking its full potential. Iodoacetic acid N-hydroxysuccinimide ester (NHS-Iodoacetate) is a prime example of a reagent whose well-defined chemical characteristics make it invaluable for numerous applications, particularly in bioconjugation and protein modification.

NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated manufacturer and supplier of fine chemicals, emphasizes the importance of these properties for our clients. Our high-purity NHS-Iodoacetate is produced with meticulous attention to detail, ensuring it meets the demanding requirements of modern research and development. When you decide to buy this compound, understanding its chemical nature empowers your scientific endeavors.

The molecular structure of NHS-Iodoacetate (C6H6INO4) features a succinimide ester activated carboxylic acid and an iodoacetate functional group. The succinimide ester is a highly reactive moiety towards primary amines, forming stable amide bonds under physiological conditions. This makes it excellent for conjugating to proteins, peptides, or amine-functionalized surfaces. The reaction is typically carried out in buffered solutions, often at a pH between 7.0 and 8.5, where the amine groups are readily available for nucleophilic attack. The byproduct, N-hydroxysuccinimide, is water-soluble and easily removed.

Concurrently, the iodoacetate group is a potent electrophile that reacts selectively with sulfhydryl (thiol) groups, primarily those found in cysteine residues of proteins. This reaction is an SN2 substitution where the sulfur atom attacks the carbon bearing the iodine, displacing iodide. The resulting thioether bond is highly stable. This selective reactivity with thiols is particularly useful for site-specific modifications, such as in the development of antibody-drug conjugates (ADCs) or the immobilization of proteins onto thiol-reactive surfaces.

The molecular weight of approximately 283.02 g/mol and its solid form (typically a yellowish to white crystalline powder) contribute to its ease of handling. Its solubility in polar organic solvents like DMF, DMSO, methanol, and dichloromethane is also advantageous for preparing reaction solutions. While less soluble in water, the reactions are often conducted in mixed solvent systems or buffered aqueous solutions where the reagent's reactivity can be effectively utilized.

For researchers aiming to buy Iodoacetic acid N-hydroxysuccinimide ester, understanding these chemical properties is crucial for optimizing conjugation strategies. Whether you are conducting protein-protein crosslinking, attaching labels, or developing novel biopharmaceuticals, the precise reactivity of NHS-Iodoacetate offers significant advantages. NINGBO INNO PHARMCHEM CO.,LTD., as a leading chemical supplier in China, provides not only the product but also the assurance of quality and consistency that your research demands.

We invite you to explore the benefits of our high-quality Iodoacetic acid N-hydroxysuccinimide ester. Contact NINGBO INNO PHARMCHEM CO.,LTD. for detailed specifications, pricing, and to arrange for samples, and let us support your critical chemical synthesis and research needs.