In the intricate world of biochemistry, maintaining the integrity of protein structures is paramount. One of the key challenges faced by researchers is the susceptibility of protein sulfhydryl groups (-SH) to oxidation, which can lead to the formation of unwanted disulfide bonds. These disulfide bonds can alter a protein's native conformation, affecting its biological activity and efficacy in experiments. This is where DL-Dithiothreitol (DTT), also known as Cleland's reagent, plays an indispensable role.

DTT is a potent small molecule redox reagent specifically designed to keep thiol groups in their reduced state. Its unique chemical structure allows it to efficiently reduce disulfide bonds and, more importantly, prevent their reformation. For scientists seeking to buy DTT, understanding its mechanisms is crucial. DTT works by undergoing an intramolecular disulfide exchange, effectively converting protein disulfides back to free thiols. This process is critical for numerous applications in molecular biology and protein science.

One of the most common uses of DTT is in sample preparation for SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Before proteins are loaded onto gels, they are often denatured. DTT is added to break any disulfide bonds, ensuring that proteins migrate solely based on their molecular weight, rather than their tertiary or quaternary structure. This leads to more accurate and reproducible results. As a leading manufacturer and supplier, we offer high-purity DTT that guarantees optimal performance in these essential techniques. If you are looking for a reliable DTT supplier in China, our product is an excellent choice.

Beyond SDS-PAGE, DTT is vital for preserving enzyme activity. Many enzymes rely on free sulfhydryl groups for their catalytic function. Oxidation can render these enzymes inactive. By including DTT in buffer solutions, researchers can effectively protect these critical functional groups. This makes DTT an invaluable component in enzyme assays and protein formulation. For those needing to purchase Cleland's reagent, ensuring high purity is key to experimental success.

DTT is also a preferred choice over other reducing agents like 2-mercaptoethanol due to its lower toxicity and odor. Furthermore, it is generally more effective at lower concentrations. When considering where to purchase DTT, choosing a reputable manufacturer ensures you receive a product that meets stringent quality standards. Our commitment to providing high-quality biochemicals makes us a trusted source for your laboratory needs. We offer competitive DTT prices and reliable supply chains, making us the go-to DTT manufacturer for researchers worldwide.