The field of microbiology relies heavily on precise identification methods to understand bacterial populations and their genetic makeup. NINGBO INNO PHARMCHEM CO.,LTD. provides essential tools for this endeavor, including the highly effective chromogenic substrate, 5-bromo-4-chloro-3-indolyl β-D (CAS: 114162-64-0). This compound plays a crucial role in the detection of Gus genes in bacterial colonies, most notably in identifying strains of E. coli.

E. coli is a common bacterium, and its presence can indicate contamination in various samples, from food and water to clinical settings. Traditional methods for identifying E. coli can be time-consuming. However, the use of chromogenic substrates like 5-bromo-4-chloro-3-indolyl β-D offers a more direct and visual approach. When E. coli, which possesses the β-glucuronidase (GUS) enzyme, encounters this substrate, it undergoes a reaction that produces a distinct blue color. This enzymatic activity allows researchers to easily differentiate Gus-positive colonies from others on a growth medium, significantly improving the efficiency of bacterial detection.

Beyond simple detection, the ability to identify Gus gene expression is fundamental in molecular biology. Researchers often use Gus gene fusions as reporter genes to study gene regulation and promoter activity in various organisms. By incorporating 5-bromo-4-chloro-3-indolyl β-D into their experimental setup, scientists can visualize where and when a particular gene is being expressed. This is particularly valuable in plant biology and genetic engineering, where confirming successful gene introduction and expression is paramount. For researchers seeking reliable Gus gene detection, the quality and consistency of the substrate are key. NINGBO INNO PHARMCHEM CO.,LTD. prides itself on supplying high-purity biochemicals that meet stringent research requirements. Our commitment as a dedicated supplier ensures that you receive products that support accurate and reproducible results in your critical studies.

The application of this substrate extends to various histochemical staining applications as well, where it aids in visualizing specific biological processes or enzyme localization within tissues. The visual output generated by the chromogenic reaction makes it an invaluable tool for both qualitative and quantitative analyses in a research setting. Whether you are focusing on microbial identification or delving into the intricacies of gene expression, leveraging effective chromogenic substrates like 5-bromo-4-chloro-3-indolyl β-D is essential for advancing scientific understanding. We are a trusted manufacturer and supplier, dedicated to providing the tools needed for successful research outcomes.