The biotechnology sector thrives on precise molecular manipulation and the development of innovative biological tools. N-Succinimidyl Bromoacetate (CAS 42014-51-7) is a key reagent that empowers these advancements, offering versatile applications in bioconjugation, protein modification, and diagnostics. This heterobifunctional crosslinker is highly valued for its ability to selectively link biomolecules, thereby enhancing their functionality and enabling novel research and development pathways.

At its core, N-Succinimidyl Bromoacetate possesses two distinct reactive sites, making it a powerful tool for creating specific molecular linkages. The succinimidyl ester end efficiently reacts with primary amine groups found on proteins, peptides, and other amine-containing molecules, forming a stable amide bond. Following this initial conjugation, the bromoacetyl moiety provides a reactive site for sulfhydryl groups, allowing for the formation of a thioether bond. This orthogonal reactivity is crucial for constructing complex bioconjugates, such as antibody-drug conjugates, targeted delivery systems, and diagnostic assays. Its typical presentation as a white powder emphasizes its role as a refined chemical product.

For professionals in the biotechnology industry, understanding the procurement of N-Succinimidyl Bromoacetate is important. When seeking to buy this reagent, it is advisable to consult with manufacturers and suppliers who can ensure high purity, often specified as ≥97% by NMR. This purity is critical for applications where specificity and minimal side reactions are essential, such as in creating diagnostic probes or therapeutic conjugates. Evaluating suppliers based on their consistency, pricing, and ability to provide necessary documentation, like CAS number 42014-51-7, is a key part of the procurement process.

The applications of N-Succinimidyl Bromoacetate in biotechnology are extensive. It is instrumental in developing fluorescently labeled antibodies for immunofluorescence and flow cytometry, creating biotinylated proteins for affinity purification and detection systems, and conjugating small molecules or peptides to proteins for therapeutic or diagnostic purposes. The reagent’s ability to form stable linkages under mild conditions makes it compatible with many sensitive biological molecules.

In summary, N-Succinimidyl Bromoacetate is an indispensable reagent for the biotechnology sector, facilitating critical molecular conjugation and modification steps. By ensuring access to high-quality products from reliable manufacturers, biotechnology companies can effectively leverage this crosslinker to drive innovation in drug discovery, diagnostics, and beyond. Its strategic importance underscores the need for careful sourcing and quality assurance when purchasing this vital chemical.