Mastering Fmoc-Asn(Trt)-OH: A Key to High-Purity Peptide Synthesis
For researchers and manufacturers deeply involved in peptide synthesis, understanding the nuances of each amino acid derivative is paramount. Among these, Fmoc-Asn(Trt)-OH stands out as a vital component for achieving high-purity peptide products. This article delves into why Fmoc-Asn(Trt)-OH is indispensable for effective peptide synthesis.
The primary challenge addressed by Fmoc-Asn(Trt)-OH is the prevention of dehydration side reactions that can occur during the activation of asparagine residues, particularly when using carbodiimide reagents. By employing the trityl (Trt) protecting group on the asparagine side chain, chemists can significantly mitigate this common issue, leading to cleaner reaction profiles and ultimately purer target peptides.
Furthermore, the solubility of Fmoc-Asn(Trt)-OH in standard peptide synthesis solvents like NMP or DMF is notably superior to that of unprotected asparagine. This improved solubility translates into more efficient coupling steps and a more streamlined synthesis process. When you buy Fmoc-Asn(Trt)-OH, you are investing in a reagent that simplifies your workflow.
The deprotection of the trityl group is typically achieved using trifluoroacetic acid (TFA). While usually complete within an hour, it's important to note that when the Asn(Trt) residue is at the N-terminal of the peptide, the deprotection time might need to be extended to two hours to ensure complete removal. This controlled deprotection is a hallmark of reliable peptide synthesis.
Researchers looking to purchase Fmoc-Asn(Trt)-OH will find it an invaluable asset. Its chemical properties, including its CAS number 132388-59-1 and molecular formula C38H32N2O5, underscore its specific utility. By integrating Fmoc-Asn(Trt)-OH into their solid phase peptide synthesis (SPPS) strategies, laboratories can consistently produce peptides with greater purity and yield, supporting breakthroughs in pharmaceutical research and development. Consider NINGBO INNO PHARMCHEM CO.,LTD. as your trusted supplier for this critical peptide synthesis reagent.
The primary challenge addressed by Fmoc-Asn(Trt)-OH is the prevention of dehydration side reactions that can occur during the activation of asparagine residues, particularly when using carbodiimide reagents. By employing the trityl (Trt) protecting group on the asparagine side chain, chemists can significantly mitigate this common issue, leading to cleaner reaction profiles and ultimately purer target peptides.
Furthermore, the solubility of Fmoc-Asn(Trt)-OH in standard peptide synthesis solvents like NMP or DMF is notably superior to that of unprotected asparagine. This improved solubility translates into more efficient coupling steps and a more streamlined synthesis process. When you buy Fmoc-Asn(Trt)-OH, you are investing in a reagent that simplifies your workflow.
The deprotection of the trityl group is typically achieved using trifluoroacetic acid (TFA). While usually complete within an hour, it's important to note that when the Asn(Trt) residue is at the N-terminal of the peptide, the deprotection time might need to be extended to two hours to ensure complete removal. This controlled deprotection is a hallmark of reliable peptide synthesis.
Researchers looking to purchase Fmoc-Asn(Trt)-OH will find it an invaluable asset. Its chemical properties, including its CAS number 132388-59-1 and molecular formula C38H32N2O5, underscore its specific utility. By integrating Fmoc-Asn(Trt)-OH into their solid phase peptide synthesis (SPPS) strategies, laboratories can consistently produce peptides with greater purity and yield, supporting breakthroughs in pharmaceutical research and development. Consider NINGBO INNO PHARMCHEM CO.,LTD. as your trusted supplier for this critical peptide synthesis reagent.
Perspectives & Insights
Molecule Vision 7
“Among these, Fmoc-Asn(Trt)-OH stands out as a vital component for achieving high-purity peptide products.”
Alpha Origin 24
“This article delves into why Fmoc-Asn(Trt)-OH is indispensable for effective peptide synthesis.”
Future Analyst X
“The primary challenge addressed by Fmoc-Asn(Trt)-OH is the prevention of dehydration side reactions that can occur during the activation of asparagine residues, particularly when using carbodiimide reagents.”