In the realm of complex organic synthesis, certain reagents stand out for their indispensable roles in facilitating specific chemical transformations. 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite is one such compound, widely recognized for its utility as a phosphitylating agent. This chemical intermediate is particularly crucial in the field of oligonucleotide synthesis, where it enables the precise construction of DNA and RNA molecules. For scientists and procurement professionals, understanding its properties and where to buy it from reliable sources is key to successful research and development projects.

The primary function of 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite, identified by its CAS number 89992-70-1, is to introduce a phosphite triester linkage. In solid-phase synthesis, it reacts with the free hydroxyl group of a nucleotide or a growing oligonucleotide chain. The N,N-diisopropylamino group renders the phosphorus atom susceptible to nucleophilic attack, while the 2-cyanoethyl ether acts as a protective group for the phosphite that can be readily removed later. This dual functionality makes it an efficient and versatile tool. When considering the purchase of this reagent, exploring options from manufacturers in China can often provide access to high-quality materials at competitive prices.

Beyond its role in nucleic acid synthesis, this phosphoramidite also finds applications in the selective monophosphorylation of carbohydrates and nucleosides. This capability is vital for researchers studying complex biological molecules and developing new synthetic pathways. The inherent reactivity and selective nature of this compound underscore its importance in advanced chemical synthesis. Therefore, when planning your next synthesis project, ensuring a reliable supply of this reagent from a reputable chemical supplier is a critical step.

The handling and storage of 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite require careful attention, as it is sensitive to moisture. Proper storage under inert atmosphere, typically argon, and at low temperatures is essential to maintain its purity and reactivity. Companies that manufacture and supply this chemical often provide detailed handling guidelines and technical support to ensure optimal usage. Understanding these requirements and sourcing from suppliers who adhere to strict quality control measures is paramount for achieving consistent and reproducible results in your synthetic endeavors.

In summary, 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite is a vital intermediate for advanced organic synthesis, particularly in the construction of DNA and RNA. By understanding its chemical properties and strategic sourcing from reliable manufacturers, researchers can effectively leverage this reagent to drive innovation in molecular biology and beyond.