The intricate field of molecular genetics often relies on specific chemical tools to unravel biological processes. For researchers working with yeast, 5-Fluoroorotic Acid (CAS 703-95-7), often referred to as 5-FOA, has become an indispensable reagent. Its unique mechanism allows for the selective screening of yeast strains, a technique vital for genetic engineering and research. This article delves into the functional role of 5-Fluoroorotic Acid in yeast genetics and provides guidance on how to effectively purchase this critical compound.

The Power of 5-Fluoroorotic Acid in Yeast Screening

At its core, the utility of 5-Fluoroorotic Acid in yeast genetics stems from a clever biochemical pathway. Orotic acid is a precursor in the de novo biosynthesis of pyrimidines, which are essential building blocks for DNA and RNA. The URA3 gene in yeast encodes for the enzyme orotidine-5′-phosphate decarboxylase. This enzyme is crucial for converting orotidine-5'-phosphate into uridine-5'-phosphate. When 5-Fluoroorotic Acid is introduced into the growth medium, and the URA3 gene is functional, the orotidine-5′-phosphate decarboxylase enzyme converts 5-Fluoroorotic Acid into the highly toxic compound 5-fluorouracil. Yeast cells that possess a functional URA3 gene will therefore be killed by 5-fluorouracil. Conversely, yeast strains that have lost or have a defective URA3 gene are resistant to 5-FOA. This resistance allows researchers to easily identify and isolate strains lacking the URA3 gene, which is fundamental for many genetic studies, including the construction of strains for gene deletion or integration experiments.

Sourcing High-Quality 5-Fluoroorotic Acid

For effective screening, the purity of 5-Fluoroorotic Acid is paramount. Researchers need to buy 5-Fluoroorotic Acid from manufacturers that guarantee a high assay, typically 98% or higher, and ensure minimal impurities that could interfere with the sensitive genetic selection process. When procuring this biochemical, it is advisable to seek out suppliers who provide detailed product specifications, including CAS number (703-95-7), molecular formula (C5H3FN2O4), and physical characteristics such as appearance (often a white to off-white powder) and melting point (around 278°C with decomposition). The origin and manufacturing standards of the supplier also play a significant role in product reliability.

Finding a Trusted Manufacturer and Supplier

As a specialized chemical supplier, NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with high-purity biochemicals. We offer 5-Fluoroorotic Acid that meets stringent quality requirements, supported by certifications and adherence to international standards. Our goal is to simplify the procurement process for scientists. We provide competitive pricing for bulk orders and welcome inquiries for product specifications and samples. If you are looking to buy 5-Fluoroorotic Acid for your yeast genetics research or other molecular biology applications, our team is ready to assist. Contact us to learn more about our product offerings and secure a reliable supply chain.