Custom DNA synthesis is a cornerstone of modern molecular biology, underpinning advancements in gene editing, diagnostics, and synthetic biology. The accuracy and efficiency of this process rely heavily on the quality of the nucleotide building blocks used. Among these, adenine phosphoramidites, particularly modified forms like DMT-dA(tac) Phosphoramidite, play a crucial role in constructing specific DNA sequences.

Understanding Nucleotide Building Blocks

DNA is a polymer composed of nucleotide monomers, each consisting of a deoxyribose sugar, a phosphate group, and a nitrogenous base (Adenine, Guanine, Cytosine, or Thymine). In the chemical synthesis of DNA, these bases are typically introduced via protected phosphoramidite derivatives. These phosphoramidites are designed to react efficiently and selectively, ensuring that the correct base is incorporated at the desired position in the growing DNA chain.

DMT-dA(tac) Phosphoramidite: Functionality and Importance

DMT-dA(tac) Phosphoramidite (CAS: 149989-58-2) is a modified adenine phosphoramidite widely used in DNA synthesis. The 'DMT' group (Dimethoxytrityl) serves as a protecting group for the 5'-hydroxyl of the nucleoside, which must be removed at the beginning of each synthesis cycle to allow for phosphoramidite coupling. The 'dA(tac)' portion refers to the adenine base, often modified for improved synthesis or stability. The 'phosphoramidite' functional group is the reactive moiety that couples to the growing DNA chain. The high purity of this compound is essential; impurities can lead to shorter, truncated DNA strands or incorrect sequences, significantly impacting downstream applications. Manufacturers strive for purity levels exceeding 98% to ensure robust and reliable DNA synthesis.

Procurement for Research and Development

For research institutions and biotech companies, securing a consistent supply of high-quality DMT-dA(tac) Phosphoramidite is vital. When seeking to buy this reagent, it is advisable to partner with established manufacturers and suppliers who specialize in nucleic acid chemistry. Companies like NINGBO INNO PHARMCHEM CO.,LTD., a prominent manufacturer and supplier in China, offer this critical component. Their expertise ensures that researchers can obtain the necessary materials for projects ranging from basic research to the development of DNA-based therapeutics.

Applications in DNA Synthesis

  • Primer Synthesis: Creating primers for PCR, qPCR, and sequencing reactions.
  • Gene Synthesis: Assembling custom gene sequences for protein expression or genetic studies.
  • Oligonucleotide Libraries: Generating diverse sets of DNA sequences for screening and discovery.
  • Diagnostic Probes: Developing highly specific probes for molecular diagnostics.

The increasing reliance on custom DNA sequences across scientific disciplines underscores the importance of reliable phosphoramidite sources. If your research or manufacturing requires DMT-dA(tac) Phosphoramidite, consider NINGBO INNO PHARMCHEM CO.,LTD. as your trusted partner for quality and supply chain integrity.