In the intricate world of peptide synthesis, the choice of reagents can significantly impact the success and efficiency of the process. Among the foundational building blocks, activated amino acid derivatives play a crucial role. This buyer's guide focuses on Boc-Leu-ONp (CAS 3350-19-4), detailing its importance, applications, and what B2B purchasers should look for when sourcing this vital compound. As a prominent chemical supplier, NINGBO INNO PHARMCHEM offers valuable perspectives on this essential peptide synthesis reagent.

Boc-Leu-ONp, scientifically known as N-Boc-L-Leucine 4-nitrophenyl ester, is a derivative of the amino acid leucine. Its structure is specifically engineered for effective use in peptide synthesis. The 'Boc' (tert-butyloxycarbonyl) group serves as a temporary protecting group for the amino terminus of leucine. This protection is vital because it prevents the free amine from reacting prematurely or forming undesired side products during the synthesis. The 'ONp' (4-nitrophenyl ester) part of the molecule activates the carboxyl terminus of leucine. This activation transforms the carboxyl group into a better leaving group, making it highly reactive towards the amino group of another amino acid or peptide chain in the coupling step.

The primary advantage of using Boc-Leu-ONp in peptide synthesis is its reliable reactivity and the relative ease of deprotection. The 4-nitrophenyl ester is a common activating group in peptide chemistry, known for its effectiveness in forming amide bonds. When a new amino acid or a growing peptide chain's N-terminus attacks the activated carboxyl carbon of Boc-Leu-ONp, a peptide bond is formed, and 4-nitrophenol is released. This reaction typically proceeds with good yields and minimal epimerization, which is critical for maintaining the stereochemical integrity of the peptide product.

For B2B buyers, understanding the specifications and application context of Boc-Leu-ONp is paramount. When you buy Boc-Leu-ONp, you are likely using it for:

  • Solid-Phase Peptide Synthesis (SPPS): Boc-Leu-ONp can be coupled to a resin-bound amino acid or peptide to extend the chain.
  • Solution-Phase Peptide Synthesis: It is also effective in solution, allowing for the synthesis of smaller peptides or peptide fragments.
  • Drug Discovery: Used in the synthesis of peptides with therapeutic potential.
  • Biochemical Research: For creating research peptides, probes, and modified proteins.

Key considerations for B2B procurement include product purity, supplier reliability, and pricing. High purity, typically ≥ 97% by HPLC, is essential to avoid unwanted side reactions and ensure the quality of the final peptide. Buyers should always look for suppliers who provide detailed Certificates of Analysis (COA) and Safety Data Sheets (SDS). For bulk purchases, manufacturers in China are often a preferred source due to their production capacity and competitive pricing structures. Engaging with experienced suppliers like NINGBO INNO PHARMCHEM ensures that you receive materials that meet industry standards and support your project timelines.

In conclusion, Boc-Leu-ONp (CAS 3350-19-4) is an indispensable reagent for anyone engaged in peptide synthesis. Its robust chemical properties facilitate efficient peptide bond formation, making it a valuable asset in drug discovery and biochemical research. By understanding its role and prioritizing reliable sourcing from qualified manufacturers, B2B professionals can ensure the success of their peptide synthesis endeavors.