The field of molecular biology and nucleic acid therapeutics is rapidly expanding, driven by advancements in synthetic biology and genetic engineering. Central to these advancements is the ability to synthesize custom DNA and RNA molecules with high fidelity. This process heavily relies on specialized chemical reagents known as phosphoramidites, which serve as the building blocks for automated synthesizers. For researchers focusing on RNA synthesis, N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, identified by CAS 121058-88-6, is an indispensable component.

The Significance of Cytidine Phosphoramidites in RNA Synthesis

Cytidine is one of the four standard nucleobases in RNA. To incorporate it into a synthetic strand using solid-phase synthesis, it must be in a protected phosphoramidite form. The specific structure of CAS 121058-88-6 features several key protecting groups designed for efficient and accurate synthesis:

  • N4-Acetyl Protection: The amino group on the cytosine base is protected by an acetyl group to prevent unwanted reactions during synthesis.
  • 2'-O-TBDMS Protection: The tert-butyldimethylsilyl (TBDMS) group on the 2'-hydroxyl position of the ribose sugar is crucial for RNA synthesis, as it stabilizes the phosphoramidite and allows for the correct phosphodiester linkage formation.
  • 5'-O-DMT Protection: The dimethoxytrityl (DMT) group on the 5'-hydroxyl is a standard protecting group that is easily removed by acidic conditions, enabling the subsequent addition of the next nucleotide.
  • Phosphoramidite Moiety: The cyanoethyl-N,N-diisopropylphosphoramidite group is the reactive center that participates in the coupling reaction.

When you choose to buy this phosphoramidite, you are investing in the precision of your RNA constructs, which are vital for applications ranging from siRNA and mRNA therapeutics to diagnostic probes.

Finding a Reliable Supplier for High-Quality Reagents

Securing a consistent supply of high-purity N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is essential for any research or manufacturing facility. As a buyer, you should look for a manufacturer and supplier that can demonstrate:

  • Commitment to Purity: Suppliers who provide detailed specifications and analytical data, such as HPLC purity and NMR spectra, are preferred. A minimum purity of 97% is generally expected for reliable synthesis.
  • Technical Expertise: A good supplier will have a deep understanding of the chemistry involved and be able to offer technical support.
  • Competitive Pricing: Especially for bulk orders, obtaining a favorable price is a significant consideration. Many suppliers in China offer cost-effective solutions for these critical reagents.
  • Prompt Delivery: Reliable shipping and handling are crucial to maintain research momentum.

For those looking to purchase this specific cytidine phosphoramidite, partnering with an experienced manufacturer in China can be highly advantageous. These companies are often equipped with advanced synthesis capabilities and can provide these essential pharmaceutical intermediates at competitive prices, making them an ideal choice for researchers and businesses alike. Ensuring a stable supply of this key component is fundamental to advancing RNA-based research and development.