The Bicinchoninic Acid (BCA) assay stands as a cornerstone in laboratories worldwide for its ability to accurately quantify total protein concentrations. At the heart of this assay lies 2,2'-Bicinchoninic Acid (CAS 1245-13-2), an organic compound whose unique chemical properties make it an indispensable reagent. As a manufacturer specializing in fine chemicals, we find immense satisfaction in supporting scientific innovation by providing high-quality 2,2'-Bicinchoninic Acid.

The magic of the BCA assay begins with a copper(II)-bicinchoninic acid complex. In an alkaline environment, peptides present in proteins reduce copper(II) ions to copper(I) ions. It is at this stage that 2,2'-Bicinchoninic Acid plays its crucial role. It acts as a highly specific chelating agent, binding to these newly formed cuprous ions. This chelation results in the formation of a vibrant, stable purple-colored complex. The intensity of this purple color is directly proportional to the concentration of copper(I) ions, and consequently, to the total protein concentration in the sample. This phenomenon is what allows for sensitive spectrophotometric detection at 562 nm.

This elegant chemical reaction underscores the importance of using high-purity 2,2'-Bicinchoninic Acid. Impurities can interfere with the reduction of copper(II) or the subsequent chelation, leading to inaccurate results. Therefore, when researchers seek to buy this reagent, choosing a supplier that guarantees its quality and purity is paramount. Our role as a manufacturer and supplier is to ensure that every batch of 2,2'-Bicinchoninic Acid (CAS 1245-13-2) we produce meets these exacting standards, providing a reliable foundation for your analytical work.

Beyond protein assays, the chelating properties of 2,2'-Bicinchoninic Acid also make it valuable in other analytical applications where metal ion detection is key. If your research involves precise measurement and you are looking to purchase this versatile organic synthesis intermediate, consider partnering with us. We offer both the quality product and the technical support you need to advance your scientific endeavors.