The field of peptide synthesis has seen remarkable advancements, largely driven by the development of sophisticated protecting group strategies and high-quality reagents. Fmoc-O-tert-Butyl-L-Threonine (CAS 71989-35-0) exemplifies this progress, serving as a crucial Fmoc-protected amino acid derivative widely utilized by scientists and manufacturers in the pharmaceutical industry. Understanding its chemical properties and applications is key to leveraging its full potential.

At its core, Fmoc-O-tert-Butyl-L-Threonine combines the well-established Fmoc (9-fluorenylmethoxycarbonyl) group with L-threonine, modified with a tert-butyl ester on its side-chain hydroxyl. This combination offers distinct advantages in solid-phase peptide synthesis (SPPS). The Fmoc group is specifically designed for facile removal under mild basic conditions (e.g., piperidine), a crucial step in chain elongation during SPPS. This orthogonal deprotection strategy avoids the harsh acidic conditions often required for Boc (tert-butyloxycarbonyl) chemistry, thereby preserving the integrity of acid-labile peptide sequences or resins.

The tert-butyl (tBu) ether on the threonine side chain is another critical feature. It protects the reactive hydroxyl group of threonine from participating in unwanted side reactions during peptide coupling. This protective group is typically cleaved during the final resin cleavage step using strong trifluoroacetic acid (TFA) cocktails, often in conjunction with scavengers. The stability afforded by the tert-butyl group ensures that the threonine residue is correctly incorporated into the growing peptide chain, maintaining the desired sequence and stereochemistry. Researchers and product developers often buy this intermediate for its predictable behavior in synthesis.

The physical and chemical characteristics of Fmoc-O-tert-Butyl-L-Threonine, such as its white crystalline powder form, melting point (typically 131-134 °C), and specific optical activity ([α]20/D +16±1°, c=1% in ethyl acetate), are indicators of its quality and suitability for demanding applications. These parameters are meticulously controlled by reputable manufacturers to ensure lot-to-lot consistency. For those in the chemical and pharmaceutical sectors seeking to buy this compound, a reliable supplier offering high purity and detailed specifications is indispensable for successful research and development.

The applications of Fmoc-O-tert-Butyl-L-Threonine are broad, including the synthesis of complex peptides for drug discovery, modification of proteins, and in various chemical biology research endeavors. Its consistent performance makes it a preferred choice for peptide manufacturers worldwide. We are dedicated to supplying this vital reagent, ensuring that your peptide synthesis projects benefit from the highest quality materials available. Reach out to us to discuss your requirements and to buy Fmoc-O-tert-Butyl-L-Threonine.