For professionals in the field of molecular biology and pharmaceutical development, understanding the underlying chemistry of essential reagents is key to optimizing synthesis processes. Thymidine Phosphoramidite (CAS 98796-51-1) is a prime example of such a reagent, serving as a foundational building block for DNA synthesis. As a dedicated chemical manufacturer, we aim to provide clarity on its structure, reactivity, and application.

The chemical name, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], reveals its intricate structure. It consists of a thymidine nucleoside, modified at the 5' position with a dimethoxytrityl (DMT) protecting group, and at the 3' position with a cyanoethyl phosphoramidite moiety. The DMT group is crucial for solubility and is readily cleaved under mild acidic conditions, while the phosphoramidite group is the reactive center for forming phosphodiester or phosphorothioate bonds during oligonucleotide synthesis.

The mechanism of action in solid-phase synthesis involves a cyclic process of deprotection, coupling, capping, and oxidation. First, the 5'-DMT group is removed, exposing the 5'-hydroxyl group. Then, the incoming Thymidine Phosphoramidite acts as a nucleophile, attacking an activated phosphoramidite on the growing oligonucleotide chain. This coupling reaction is typically facilitated by an activating agent, such as tetrazole or its derivatives. The resulting phosphite triester is then oxidized to a more stable phosphate triester, completing one cycle of addition.

As a leading supplier of DNA synthesis building blocks, we ensure our Thymidine Phosphoramidite is synthesized under stringent conditions to maintain its high purity and reactivity. This consistent quality is vital for achieving high coupling efficiencies and producing full-length oligonucleotides, minimizing truncation sequences.

For researchers and procurement specialists looking to buy Thymidine Phosphoramidite, understanding these chemical principles underscores the importance of sourcing from a reliable manufacturer in China. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing intermediates like this CAS 98796-51-1 product with the chemical integrity required for demanding applications in molecular biology and drug discovery. We invite you to learn more about our quality standards and how we can support your synthesis needs.