Choosing the Right Biological Buffer: MES Sodium Salt Explained
In the realm of biological and biochemical research, maintaining a stable pH is paramount. Researchers frequently rely on buffering agents to achieve this critical stability. Among the wide array of available buffers, MES Sodium Salt (CAS 71119-23-8) stands out due to its unique zwitterionic nature and advantageous properties. As a leading manufacturer and supplier in China, NINGBO INNO PHARMCHEM CO.,LTD. aims to shed light on why this compound is an indispensable tool for scientists worldwide.
MES Sodium Salt, scientifically known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, belongs to the Good's buffers family. These buffers were specifically designed in the 1960s to meet stringent criteria for biological research, including a stable pKa, high water solubility, minimal salt effects, and chemical inertness. MES Sodium Salt perfectly embodies these characteristics, offering a robust buffering capacity within the pH range of 5.5 to 7.0, with a pKa of approximately 6.15 at 20°C. This pH range is particularly relevant for many biological processes, making it a highly sought-after reagent.
One of the significant advantages of MES Sodium Salt is its minimal absorption in both the UV and visible spectral ranges. This property is invaluable for researchers conducting spectrophotometric analyses, as it reduces the risk of interference and ensures more accurate data. Furthermore, MES Sodium Salt exhibits weak binding with several metal ions, including calcium (Ca), magnesium (Mg), and manganese (Mn), and negligible binding with copper (Cu(II)). This makes it an excellent choice for experiments involving metal ions where traditional buffers like phosphate or acetate might form undesirable complexes.
The versatility of MES Sodium Salt is evident in its widespread applications. It is frequently employed as a biological buffer in plant cell culture, supporting healthy growth and development. In molecular biology and biochemistry, it serves as an effective running buffer for electrophoresis, particularly for resolving small proteins on Bis-Tris gels. Its utility extends to various chromatography techniques, such as cation-exchange chromatography, where it aids in protein purification. For those seeking to buy MES sodium salt, understanding these applications highlights its importance in achieving reproducible and reliable experimental outcomes.
At NINGBO INNO PHARMCHEM CO.,LTD., we pride ourselves on being a dependable MES Sodium Salt manufacturer and supplier. We offer high-purity MES Sodium Salt at competitive prices, catering to both academic institutions and industrial laboratories. Our commitment is to provide researchers with the essential tools they need to push the boundaries of scientific discovery. If you are looking to procure high-quality MES Sodium Salt, we encourage you to contact us for a quote and sample to experience the difference our product and service can make in your research endeavors.
Perspectives & Insights
Agile Reader One
“In molecular biology and biochemistry, it serves as an effective running buffer for electrophoresis, particularly for resolving small proteins on Bis-Tris gels.”
Logic Vision Labs
“Its utility extends to various chromatography techniques, such as cation-exchange chromatography, where it aids in protein purification.”
Molecule Origin 88
“For those seeking to buy MES sodium salt, understanding these applications highlights its importance in achieving reproducible and reliable experimental outcomes.”