In the intricate world of biochemical analysis and diagnostics, the selection of the right substrate is paramount for achieving accurate and reliable results. Chromogenic substrates, in particular, play a pivotal role by providing a visual cue—color—that indicates the presence and activity of specific enzymes. Among the widely used compounds in this category is 2-Nitrophenyl Beta-D-Galactopyranoside (CAS 369-07-3), a key player in assays involving β-galactosidase. As a leading manufacturer and supplier based in China, we are dedicated to providing high-quality chromogenic substrates to the global scientific community.

What is a Chromogenic Substrate?

A chromogenic substrate is a substance that reacts with a specific enzyme to produce a colored product. This color change allows for the easy detection and quantification of enzyme activity without the need for complex detection equipment, making assays more accessible and cost-effective. The specificity of the substrate ensures that the observed color is a direct result of the target enzyme's action, minimizing interference from other biological components.

2-Nitrophenyl Beta-D-Galactopyranoside: A Versatile Tool

2-Nitrophenyl Beta-D-Galactopyranoside (2-NPG) is a prime example of a successful chromogenic substrate. It is specifically designed to be hydrolyzed by the enzyme β-galactosidase. Upon cleavage, the β-galactosidase enzyme releases a p-nitrophenol moiety, which, in an alkaline environment, turns yellow. The intensity of this yellow color is directly proportional to the amount of β-galactosidase present. This property makes 2-NPG indispensable for:

  • Enzyme kinetics studies: Measuring reaction rates and enzyme efficiency.
  • Reporter gene assays: Indicating gene expression levels.
  • Microbiological testing: Identifying specific bacteria strains.
  • Quality control in food and beverage industries: Detecting microbial contamination.

For researchers and manufacturers, the ability to buy 2-Nitrophenyl Beta-D-Galactopyranoside with high purity (≥97% or higher) and consistent quality is essential. As a specialized manufacturer and supplier in China, we understand these critical needs. Our product, identified by CAS 369-07-3, is produced under stringent quality control measures to ensure optimal performance in your applications.

Why Choose a China-Based Manufacturer for Your Substrates?

The global chemical supply chain benefits immensely from the robust manufacturing capabilities in China. When you choose to buy chromogenic substrates like 2-NPG from us, you gain access to:

  • Cost-Effectiveness: Our manufacturing efficiency allows us to offer competitive pricing without compromising on quality.
  • Reliable Supply: We maintain consistent production and inventory to ensure you have a stable supply of critical reagents.
  • Technical Expertise: Our team possesses deep knowledge in chemical synthesis and quality assurance, ensuring product integrity.
  • Scalability: We can accommodate orders ranging from grams for research to kilograms for industrial production.

We are committed to supporting your scientific endeavors and industrial processes. Whether you are developing new diagnostic kits, conducting academic research, or implementing quality control measures, our 2-Nitrophenyl Beta-D-Galactopyranoside is manufactured to meet your highest expectations. We invite you to connect with us to discuss your specific requirements and explore how our high-quality chromogenic substrates can enhance your work.