Bioluminescence assays have revolutionized our ability to study biological processes in real-time and with exceptional sensitivity. At the core of many of these powerful techniques is Coelenterazine (CAS 55779-48-1), a naturally occurring luciferin. NINGBO INNO PHARMCHEM CO.,LTD. is a dedicated manufacturer and supplier committed to providing researchers with this high-value compound for their diverse assay needs.

Coelenterazine is renowned for its function as a substrate for a variety of luciferase enzymes, including Renilla and Gaussia luciferases, and photoproteins like aequorin. The light emitted upon oxidation by these enzymes allows for quantitative measurements of biological phenomena. Researchers often purchase Coelenterazine to implement these assays due to its efficiency and broad applicability.

One of the primary applications of Coelenterazine is in reporter gene assays. By coupling the expression of a target gene to the activity of a luciferase, the light output directly correlates with the gene's expression level. This method is widely used in drug discovery, genetic research, and cellular pathway analysis. Securing a consistent and high-purity supply of Coelenterazine from a reputable manufacturer like NINGBO INNO PHARMCHEM is vital for obtaining reproducible results.

Another critical application is in the measurement of intracellular calcium levels. The aequorin photoprotein complex, when reconstituted with Coelenterazine, exhibits bioluminescence that is directly proportional to intracellular calcium concentration. This technique is invaluable for studying cellular signaling, muscle contraction, and neuronal activity. Researchers seeking to buy Coelenterazine for these sophisticated measurements rely on suppliers who guarantee product integrity.

Furthermore, Coelenterazine serves as a valuable tool for detecting reactive oxygen species (ROS), such as superoxide anions, in biological systems. Its chemiluminescent properties allow for sensitive quantification of oxidative stress, which is implicated in numerous diseases. The accessibility of Coelenterazine from reliable China manufacturers like us simplifies the implementation of these important analytical methods.

NINGBO INNO PHARMCHEM CO.,LTD. prides itself on being a premier manufacturer and supplier of Coelenterazine. We understand the demands of modern biological research and are committed to offering this essential biochemical with guaranteed purity and competitive pricing. If you are in need of Coelenterazine for your bioluminescence assays, we invite you to contact us to discuss your requirements and experience the difference of working with a dedicated supplier.