Flow cytometry is a powerful technique used to analyze the physical and chemical characteristics of particles, most commonly cells, as they flow through a laser beam. Central to many flow cytometry applications is the ability to quantify cellular DNA content. 4',6-Diamidino-2-phenylindole Dihydrochloride (DAPI) is a fluorescent dye that has become a critical reagent for DNA staining in flow cytometry, enabling detailed cell cycle analysis and quantitative studies. For institutions looking to buy DAPI for these purposes, understanding its role is essential.

DAPI's effectiveness in flow cytometry stems from its specific DNA-binding properties and its fluorescence characteristics. When introduced to cells and excited by a suitable laser (typically UV), DAPI exhibits a strong blue fluorescence emission proportional to the amount of DNA present in the cell. Cells in different phases of the cell cycle possess varying amounts of DNA. For instance, cells in G1 phase have a diploid DNA content (2N), while cells in G2/M phase have twice that amount (4N). Cells undergoing DNA replication in the S phase have an intermediate DNA content.

By quantitatively measuring the fluorescence intensity of DAPI-bound cells, flow cytometry can generate histograms that depict the distribution of cells across the cell cycle. Researchers can identify distinct peaks corresponding to the G1 and G2/M phases, with the cells in between representing the S phase. This information is invaluable for studying cell proliferation, understanding the effects of drugs or genetic manipulations on cell division, and assessing cellular health.

Beyond cell cycle analysis, DAPI is also used in flow cytometry for ploidy analysis, chromosome sorting, and as a viability dye. In viability studies, DAPI is often used because it can enter cells with compromised membranes (dead or damaged cells) but is largely excluded by intact, live cell membranes. This allows for the differentiation of live and dead cell populations within a sample. For these demanding applications, acquiring high-purity DAPI from a reliable supplier is crucial for accurate and reproducible data.

As a dedicated manufacturer and supplier of DAPI, we ensure that our product meets the stringent requirements for flow cytometry applications. Our commitment to quality means that researchers can buy DAPI with confidence, knowing they are using a reagent that provides consistent and reliable DNA staining for precise cell cycle analysis and viability assessment. We cater to the needs of research laboratories worldwide, offering competitive pricing and dependable supply to support your critical scientific investigations.