In the realm of advanced pharmaceutical formulations, particularly those involving nucleic acid delivery, the role of specialized lipids cannot be overstated. 1,2-Dioleoyloxy-3-(dimethylamino)propane, commonly known as DODAP, stands out as a critical ionizable cationic lipid. Its unique physicochemical properties make it indispensable for creating effective delivery systems such as lipid nanoparticles (LNPs) and liposomes. For procurement managers and R&D scientists looking to buy DODAP, understanding its synthesis, properties, and the benefits of sourcing from a reliable DODAP supplier in China is essential for successful project outcomes.

The Chemistry and Functionality of DODAP

DODAP is synthesized with two oleoyl (oleic acid) ester chains attached to a glycerol backbone, with a dimethylamino group at the sn-3 position. This structure imbues DODAP with amphipathic characteristics. The long, unsaturated oleoyl chains provide hydrophobicity, while the tertiary amine group offers a hydrophilic and, crucially, ionizable center. This ionizability means DODAP is neutral at physiological pH but becomes positively charged when protonated in acidic environments (typically pH below its pKa of around 8.02). This pH-dependent charge is the cornerstone of its utility in intracellular delivery.

When DODAP is incorporated into LNPs or liposomes, it plays a dual role:

  • Nucleic Acid Binding: At slightly acidic pH during formulation, DODAP can interact electrostatically with negatively charged nucleic acids like mRNA or siRNA, facilitating their entrapment.
  • Endosomal Escape: Upon cellular uptake and entry into endosomes, the acidic pH causes DODAP to become cationic. This positive charge disrupts the endosomal membrane, promoting the release of the nucleic acid payload into the cell cytoplasm, thereby enhancing transfection efficiency.

Its relatively low cytotoxicity and ability to form stable particles further solidify its position as a preferred cationic lipid in many cutting-edge formulations, including those for mRNA vaccines and gene therapies.

Synthesis and Quality Considerations

The synthesis of high-purity DODAP involves esterification reactions and careful purification steps to remove impurities that could compromise its performance or safety. For pharmaceutical applications, achieving a purity exceeding 98% is often a prerequisite. When procuring DODAP, it's vital to work with a DODAP manufacturer that adheres to stringent quality control protocols and can provide comprehensive documentation, including Certificates of Analysis (CoA). Understanding the DODAP price in relation to its purity and the supplier's reliability is a key procurement strategy.

Why Partner with a Chinese Supplier?

As global supply chains evolve, many pharmaceutical companies are looking to China for high-quality chemical intermediates. NINGBO INNO PHARMCHEM CO.,LTD. is a premier DODAP supplier in China, offering:

  • Consistent High Purity: Ensuring batch-to-batch uniformity essential for reproducible results.
  • Competitive Pricing: Providing cost-effective solutions without compromising quality.
  • Reliable Supply: A stable manufacturing base to meet demand for research and commercial production.

We invite you to purchase DODAP from NINGBO INNO PHARMCHEM CO.,LTD. for your advanced formulation needs. Contact us today to discuss your requirements and receive a personalized quote.