Exploring DPPH's Versatility: From Antioxidant Assays to Spectroscopic Standards
In the dynamic field of chemical research, certain compounds emerge as linchpins due to their unique properties and broad applicability. DPPH, known chemically as 2,2-Diphenyl-1-picrylhydrazyl and identified by CAS number 1898-66-4, is one such compound. As a stable free radical, it possesses a distinct deep violet color that readily changes upon reduction, making it an exceptionally versatile tool for a variety of laboratory applications.
Perhaps the most renowned use of DPPH is in the quantitative assessment of antioxidant activity. The compound functions as an effective DPPH free radical scavenger, readily accepting a hydrogen atom from antioxidant compounds. This reaction leads to the decolorization of DPPH, a change that can be easily measured via spectrophotometry. This forms the basis of the widely employed antioxidant assay DPPH, which allows scientists to screen and rank the antioxidant potential of various natural extracts, synthetic compounds, and food products. The reliability of DPPH in these assays is critical for researchers in fields such as nutraceuticals, pharmaceuticals, and food science who are constantly seeking compounds that can mitigate oxidative damage.
Beyond its celebrated role in antioxidant studies, DPPH serves as a valuable monitor for chemical reactions involving free radicals. Its colorimetric transformation acts as a visual cue, indicating the progression of radical reactions. This makes it an essential reagent for chemists investigating reaction kinetics and mechanisms. The compound's stability also allows it to function as a benchmark or standard in Electron Paramagnetic Resonance (EPR) spectroscopy. As an electron paramagnetic resonance (EPR) signal standard, DPPH provides a consistent reference point for calibrating EPR instruments, ensuring the accuracy and comparability of spectral data across different experimental setups.
The DPPH CAS 1898-66-4 uses also extend to specific analytical determinations. It is utilized in the indirect spectroscopic determination of thiols, which are important in biological systems and industrial processes. Furthermore, its application in the photometric determination of tocopherol (Vitamin E) highlights its utility in analyzing biologically relevant compounds. The consistent behavior of DPPH as a hydrogen radical scavenger compound across these diverse applications underscores its importance in scientific research and development.
NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with premium quality DPPH, ensuring the accuracy and reliability needed for groundbreaking discoveries. By offering this essential reagent, we aim to support advancements in understanding and combating oxidative stress and exploring the complexities of radical chemistry.
Perspectives & Insights
Agile Reader One
“The compound's stability also allows it to function as a benchmark or standard in Electron Paramagnetic Resonance (EPR) spectroscopy.”
Logic Vision Labs
“As an electron paramagnetic resonance (EPR) signal standard, DPPH provides a consistent reference point for calibrating EPR instruments, ensuring the accuracy and comparability of spectral data across different experimental setups.”
Molecule Origin 88
“It is utilized in the indirect spectroscopic determination of thiols, which are important in biological systems and industrial processes.”