The precise analysis and synthesis of peptides are fundamental to advancements in pharmaceuticals, diagnostics, and biochemical research. Fluorescamine (CAS: 38183-12-9) plays a critical role in these processes by enabling highly sensitive detection and quantification of primary amines, which are abundant in peptides. As a leading manufacturer and supplier of specialty chemicals, we provide high-quality Fluorescamine to support these vital scientific endeavors. If you are considering where to buy this essential reagent, our competitive pricing and reliable supply chain from China make us an ideal partner.

Understanding Fluorescamine's Role in Peptide Chemistry

Fluorescamine is a non-fluorescent compound that reacts selectively and rapidly with primary aliphatic and aromatic amines under mild conditions. This reaction yields a stable, highly fluorescent derivative, allowing for the detection of even minute quantities of amines. In the context of peptides, which are chains of amino acids linked by peptide bonds, the presence of free amino groups (e.g., at the N-terminus or on side chains of certain amino acids) makes them ideal targets for Fluorescamine derivatization.

Applications in Peptide Synthesis and Analysis:

  • N-terminal Analysis: Fluorescamine can be used to identify and quantify the N-terminal amino acid of a peptide. This is crucial for confirming the integrity of synthesized peptides and for sequencing.
  • Quantification of Peptides: By reacting with the free amino groups, Fluorescamine allows for the accurate spectrophotometric or spectrofluorometric determination of peptide concentrations in solution. This is vital for preparing accurate concentrations for subsequent experiments.
  • Monitoring Peptide Synthesis: The reagent can be used to monitor the progress of solid-phase peptide synthesis (SPPS) by detecting unreacted primary amines or by quantifying newly formed peptide chains.
  • Detection of Amines in Peptide Libraries: In combinatorial chemistry and the screening of peptide libraries, Fluorescamine provides a sensitive method for identifying active compounds or verifying synthesis success.

The efficiency and sensitivity of Fluorescamine make it a preferred choice over many other derivatization agents. Its reaction is fast, requires minimal incubation, and the resulting fluorescent products are stable, minimizing experimental variability. Furthermore, the low background noise associated with Fluorescamine ensures high signal-to-noise ratios, enabling precise measurements even for complex peptide mixtures.

For laboratories and chemical companies engaged in peptide research and development, securing a consistent and high-quality source of Fluorescamine is paramount. Our company, a dedicated supplier based in China, offers Fluorescamine (CAS 38183-12-9) with guaranteed purity and competitive prices. We are committed to supporting scientific innovation by providing reliable chemical building blocks. If you need to purchase Fluorescamine for your peptide synthesis or analytical needs, please reach out to us for a quote and to learn more about our product offerings and bulk purchase options.