In the dynamic field of peptide research, the ability to accurately and reliably synthesize specific peptide sequences is paramount. Cysteine, with its unique thiol group, poses a challenge that is elegantly solved by using protected amino acid derivatives. Among these, Fmoc-Cys(Trt)-OH has emerged as a widely adopted and highly effective reagent. This article highlights why it is a favored choice for researchers and where to procure it from dependable sources.

Why Fmoc-Cys(Trt)-OH is Preferred

The versatility of cysteine in forming disulfide bonds and its potential for nucleophilic reactions make its side chain protection crucial during peptide synthesis. Fmoc-Cys(Trt)-OH offers a dual protection strategy that is highly advantageous:

  • Fmoc Protection: The N-terminal Fmoc group is the standard for base-labile Fmoc-based solid-phase peptide synthesis (SPPS), allowing for iterative chain elongation.
  • S-Trityl Protection: The trityl (Trt) group attached to the thiol of cysteine is a sterically bulky and acid-labile protecting group. It effectively prevents unwanted oxidation, alkylation, or disulfide bond formation during peptide chain assembly. Importantly, it can be selectively cleaved using mild acidic conditions, often in the final deprotection step, without affecting other acid-labile protecting groups on the peptide.

This combination ensures that cysteine residues are incorporated accurately into the peptide sequence, preserving their intended functionality for downstream research applications.

Key Specifications and Quality Considerations for Buyers

When researchers decide to buy Fmoc-Cys(Trt)-OH, they prioritize high quality. The typical specifications are:

  • Purity: ≥99.0% by HPLC. This ensures minimal side products and efficient coupling.
  • Chemical Identity: Verified by NMR and Mass Spectrometry.
  • CAS Number: 103213-32-7.
  • Molecular Formula: C37H31NO4S.
  • Molecular Weight: Approximately 585.71 g/mol.

The reliable performance of Fmoc-Cys(Trt)-OH in peptide research makes it a sought-after reagent. It's a fundamental component for synthesizing peptides used in drug development, protein engineering, and various biochemical assays.

Reliable Sourcing from Manufacturers

For laboratories and research institutions, sourcing such critical reagents from trusted manufacturers is paramount. We specialize in providing high-purity Fmoc-Cys(Trt)-OH, ensuring that your peptide research is supported by reagents of the highest quality. By choosing us as your supplier, you benefit from our commitment to excellence in chemical manufacturing and our understanding of the needs of the peptide research community. Explore our offerings to ensure your next peptide synthesis project is a success.