In the dynamic field of peptide synthesis, the availability of high-quality, precisely functionalized building blocks is paramount for achieving successful outcomes. Fmoc-O-(benzylphospho)-L-threonine, identified by its CAS number 175291-56-2, is a prime example of such an advanced chemical intermediate, offering researchers the ability to incorporate phosphorylated threonine residues into peptide chains. This capability is crucial for studying protein phosphorylation, a fundamental biological process involved in signal transduction, gene regulation, and numerous cellular functions.

The significance of Fmoc-O-(benzylphospho)-L-threonine lies in its role as a key component in solid-phase peptide synthesis (SPPS), particularly when employing the Fmoc/tBu strategy. The Fmoc protecting group is selectively removed under mild basic conditions, allowing for efficient stepwise addition of amino acids to the growing peptide chain. The benzylphospho moiety on the threonine side chain can be selectively deprotected later in the synthesis, usually via hydrogenolysis or acid cleavage, to reveal the free phosphate group. This controlled introduction of phosphorylation is essential for creating phosphopeptides that accurately mimic their native biological counterparts, which is vital for drug discovery, enzyme studies, and the development of diagnostic tools.

For researchers and procurement specialists looking to buy Fmoc-O-(benzylphospho)-L-threonine, understanding the importance of purity cannot be overstated. Impurities can lead to truncated peptides, undesired side products, and significantly impact the overall yield and success of the synthesis. Therefore, it is crucial to source this intermediate from a reliable manufacturer or supplier that guarantees high purity, typically ≥98% by HPLC. When evaluating potential vendors, always request detailed product specifications and Certificates of Analysis (CoAs) to confirm the quality and consistency of the material.

Sourcing specialized pharmaceutical intermediates like Fmoc-O-(benzylphospho)-L-threonine from international markets, such as China, can provide access to a wide range of products at competitive price points. However, it is essential to conduct thorough due diligence on potential suppliers. Look for companies with established quality management systems, a proven track record in chemical synthesis, and excellent customer service. Obtaining multiple quotes and comparing supplier capabilities will help ensure you secure both quality and value for your research needs.

The applications of phosphopeptides synthesized using Fmoc-O-(benzylphospho)-L-threonine are diverse, spanning areas like kinase inhibitor development, studying protein-protein interactions mediated by phosphorylation, and creating custom peptide-based therapeutics. By providing access to high-purity building blocks, we aim to support these critical research endeavors. We encourage you to reach out to us for your requirements of Fmoc-O-(benzylphospho)-L-threonine. Partnering with a trusted supplier ensures that your peptide synthesis projects are built on a foundation of quality and reliability.