Guanidine Hydrochloride (CAS 50-01-1) is celebrated in biochemical and molecular biology laboratories for its potent denaturing capabilities. As a strong chaotropic agent, it plays a crucial role in unfolding proteins, essential for various research techniques. For scientists and lab managers aiming to achieve reliable experimental outcomes, understanding its application in protein denaturation and sourcing from a trusted supplier is fundamental.

The Power of Guanidine Hydrochloride in Protein Denaturation

Proteins, with their intricate three-dimensional structures, are stabilized by a network of non-covalent interactions, including hydrogen bonds and hydrophobic forces. Guanidine Hydrochloride, typically used at high molarities (e.g., 6 M), effectively disrupts these forces. It increases the solubility of hydrophobic amino acid side chains and interferes with hydrogen bonding networks, causing proteins to lose their native conformation and unfold into linear polypeptide chains. This process is vital for solubilizing proteins, especially those aggregated in inclusion bodies, and for preparing samples for techniques like electrophoresis or mass spectrometry.

Applications in Research and Diagnostics

The denaturing property of Guanidine Hydrochloride makes it invaluable for numerous research applications. In protein purification, it helps to extract and maintain proteins in a soluble state. It is also a critical component in protocols for nucleic acid extraction, where its ability to disrupt cell membranes and denature proteins aids in isolating DNA and RNA. Researchers frequently buy Guanidine Hydrochloride online for these sensitive biological assays, prioritizing high purity and consistent performance. Ensuring you source from a reliable Guanidine Hydrochloride supplier guarantees the integrity of your experimental results.

Sourcing Quality Guanidine Hydrochloride

When seeking Guanidine Hydrochloride for laboratory use, it is imperative to choose a supplier that guarantees purity and quality. A reputable Guanidine Hydrochloride CAS 50-01-1 manufacturer will provide detailed specifications, including analysis of critical parameters like UV absorbance at 260nm and 280nm, which are important for nucleic acid work. Understanding the competitive Guanidine Hydrochloride price in China can assist in budget planning for research institutions. For bulk procurement, exploring options for wholesale Guanidine Hydrochloride for protein denaturation ensures a cost-effective supply.

NINGBO INNO PHARMCHEM CO.,LTD. is a premier manufacturer and supplier of Guanidine Hydrochloride, dedicated to serving the scientific community. We offer high-purity Guanidine Hydrochloride essential for protein denaturation and other critical laboratory applications. We invite you to contact us for product inquiries and to secure your supply from a trusted source.