Hematoxylin and Eosin Staining: The Foundation of Tissue Analysis
The Hematoxylin and Eosin (H&E) stain is arguably the most fundamental and widely used staining technique in histopathology and histology laboratories worldwide. This powerful combination relies on the precise interplay between two key dyes: Hematoxylin, which stains nuclei blue-black, and Eosin, which stains cytoplasm and extracellular matrix pink. NINGBO INNO PHARMCHEM CO.,LTD. is a leading provider of high-quality Hematoxylin (CAS 517-28-2), offering a pure product essential for the success of H&E staining.
Hematoxylin, derived from the logwood tree, acts as a nuclear stain. Its effectiveness stems from its ability to form a colored complex with metallic mordants, typically aluminum salts. This complex, known as a hemalum stain, binds to acidic components within the nucleus, such as DNA and RNA. The resulting vibrant blue-black color highlights the nuclear structure, including the chromatin and nucleolus, providing crucial diagnostic information. The chemical formula of Hematoxylin is C16H14O6, and its purity of 99% from NINGBO INNO PHARMCHEM CO.,LTD. ensures consistent performance in H&E protocols.
Eosin, on the other hand, is an acidic dye that stains the cytoplasm, collagen, muscle fibers, and other basic cellular components in various shades of pink to red. The contrast achieved by the combined Hematoxylin and Eosin staining is invaluable for differentiating various tissue types and identifying cellular morphology. This dual staining method allows pathologists to assess everything from normal tissue architecture to the subtle changes indicative of disease. For laboratories seeking to buy Hematoxylin and reliable staining solutions, NINGBO INNO PHARMCHEM CO.,LTD. provides the foundational component for this critical technique.
The reliability of the H&E stain is largely attributed to the consistent quality of its constituent dyes. Sourcing high-purity Hematoxylin powder is crucial for laboratories to maintain the accuracy and reproducibility of their staining. Our commitment at NINGBO INNO PHARMCHEM CO.,LTD. is to provide laboratories with precisely that: high-purity Hematoxylin powder that performs exceptionally well in H&E staining, ensuring that every slide provides clear and meaningful diagnostic information. When you buy Hematoxylin from us, you are choosing a partner dedicated to the quality of your results.
In essence, the Hematoxylin and Eosin stain is the bedrock of tissue analysis. Understanding the role of each component, particularly the precise nuclear staining provided by high-quality Hematoxylin, is key to its successful application. NINGBO INNO PHARMCHEM CO.,LTD. is proud to supply this vital reagent, supporting the critical work of histopathologists and researchers worldwide.
Perspectives & Insights
Molecule Vision 7
“Eosin, on the other hand, is an acidic dye that stains the cytoplasm, collagen, muscle fibers, and other basic cellular components in various shades of pink to red.”
Alpha Origin 24
“The contrast achieved by the combined Hematoxylin and Eosin staining is invaluable for differentiating various tissue types and identifying cellular morphology.”
Future Analyst X
“This dual staining method allows pathologists to assess everything from normal tissue architecture to the subtle changes indicative of disease.”