Polyinosinic:Polycytidylic Acid (Poly I:C) is a cornerstone reagent in modern immunology, valued for its ability to reliably activate innate immune pathways. Its synthetic nature and structural similarity to viral double-stranded RNA (dsRNA) make it an indispensable tool for both in vitro cellular studies and in vivo animal experiments. Researchers worldwide depend on a consistent supply of high-quality Poly I:C to advance their investigations.

In Vitro Applications:

In laboratory settings, Poly I:C is extensively used to stimulate immune cells and investigate signaling pathways. Common applications include:

  • TLR3 Activation Assays: Poly I:C is the gold standard agonist for studying Toll-like Receptor 3 (TLR3) signaling. Researchers use it to examine downstream effects such as cytokine production (e.g., IFN-β, TNF-α), NF-κB activation, and changes in gene expression in various cell types, including dendritic cells, macrophages, and epithelial cells.
  • Antiviral Response Studies: By triggering innate immune responses, Poly I:C can be used to mimic viral infections in cell culture, allowing scientists to study cellular defense mechanisms and the efficacy of potential antiviral compounds.
  • Immune Cell Differentiation and Maturation: Poly I:C has been shown to promote the maturation of dendritic cells and influence the differentiation of other immune cell populations, making it valuable for immunophenotyping and functional studies.

In Vivo Applications:

The use of Poly I:C in animal models offers a more comprehensive understanding of immune responses in a complex biological system:

  • Immune System Modulation: Administering Poly I:C to animals is a common method for systemically activating the innate immune system. This is used to study inflammatory processes, host defense mechanisms against pathogens, and the potential of Poly I:C as a vaccine adjuvant to boost adaptive immunity.
  • Disease Modeling: Poly I:C has been employed to establish animal models for various conditions, including neuroinflammation, autoimmune disorders, and models simulating viral infections. For example, it is used to study maternal immune activation and its potential impact on offspring neurodevelopment.
  • Cancer Immunotherapy Research: Its ability to activate antigen-presenting cells and induce cytokine production makes Poly I:C a subject of interest in cancer immunotherapy research, often explored as a component in combination therapies to enhance anti-tumor immunity.

Given these critical applications, sourcing reliable Poly I:C is paramount. When you look to buy Poly I:C, ensure you partner with a reputable Poly I:C supplier. We are a leading Poly I:C manufacturer in China, committed to providing high-purity Polyinosinic:Polycytidylic Acid for both in vitro and in vivo research. Our rigorous quality control ensures that you receive a consistent and effective product. Contact us to inquire about Poly I:C price and availability to support your groundbreaking research.