In the dynamic field of biotechnology and pharmaceutical research, the ability to precisely link different biomolecules is paramount. This process, known as bioconjugation, underpins advancements in diagnostics, therapeutics, and fundamental biological studies. At the heart of many successful bioconjugation strategies lies the use of sophisticated crosslinking reagents. Among the most versatile and widely employed is 3-Maleimidobenzoic acid N-hydroxysuccinimide ester, commonly referred to as MBS.

As a trusted manufacturer and supplier of high-quality chemical intermediates in China, we understand the critical role that reliable reagents play in your research and production. This article delves into the properties and applications of MBS, guiding researchers on how to effectively buy and utilize this essential tool for their bioconjugation needs.

Understanding MBS: A Heterobifunctional Powerhouse

MBS is a heterobifunctional crosslinker, meaning it possesses two distinct reactive groups designed to react with different types of functional groups on biomolecules. Specifically, MBS features:

  • N-Hydroxysuccinimide (NHS) Ester: This moiety is highly reactive towards primary amine groups (-NH2) found on lysine residues and the N-terminus of proteins and peptides. The reaction typically occurs under slightly alkaline conditions (pH 7-9).
  • Maleimide Group: This group exhibits high specificity for sulfhydryl groups (-SH), commonly found in cysteine residues of proteins. This reaction is most efficient in the pH range of 6.5-7.5.

The combination of these two reactive ends, separated by an aromatic spacer, allows MBS to bridge molecules with amine and sulfhydryl functionalities, creating stable, covalent linkages. The non-cleavable nature of the MBS spacer arm is a significant advantage, ensuring the integrity of the conjugate throughout its lifecycle.

Why Choose MBS? Key Applications

The versatility of MBS makes it an indispensable reagent across numerous scientific disciplines. Researchers and product developers frequently buy MBS for the following applications:

Protein Modification and Labeling

One of the primary uses of MBS is in modifying proteins. By attaching labels such as fluorescent dyes, biotin, or enzymes to specific sites on a protein, researchers can enhance detection sensitivity, track protein localization within cells, or develop targeted assays. The ability to selectively react with amines or thiols allows for controlled labeling, minimizing interference with the protein's native function.

Antibody-Drug Conjugates (ADCs)

In the realm of targeted cancer therapy, ADCs represent a significant breakthrough. MBS plays a crucial role in linking a potent cytotoxic drug to a monoclonal antibody that specifically targets cancer cells. This targeted delivery system minimizes systemic toxicity. If you are involved in ADC development, purchasing high-purity MBS from a reliable manufacturer like us is a critical step towards successful drug conjugation.

Hapten-Carrier Conjugation

For the development of immunoassays, vaccines, and diagnostic tools, small molecules (haptens) often need to be conjugated to larger carrier proteins. This process enhances the immunogenicity of the hapten. MBS is an efficient reagent for creating these hapten-carrier conjugates, ensuring robust immune responses or accurate detection signals.

Enzyme Immobilization

The immobilization of enzymes onto solid supports or surfaces is vital for industrial biocatalysis and biosensor development. MBS can be used to covalently link enzymes to functionalized materials, providing stable and reusable biocatalytic systems. We offer MBS for bulk purchase, supporting large-scale industrial applications.

Sourcing High-Quality MBS: Your Trusted Partner in China

When it comes to crucial reagents like MBS, quality and reliability are non-negotiable. As a leading chemical supplier in China, we are dedicated to providing researchers and manufacturers with the highest purity MBS available. Our stringent quality control processes ensure that every batch meets rigorous standards, giving you the confidence to proceed with your experiments and production without compromise. We understand that timely supply is also critical, which is why we maintain robust inventory and efficient logistics.

For researchers looking to buy MBS for their next breakthrough, or for companies seeking a dependable supplier for their manufacturing needs, we offer competitive pricing and excellent customer support. Explore our product catalog and discover how our commitment to quality can empower your scientific endeavors. Contact us today to inquire about pricing and bulk purchase options for 3-Maleimidobenzoic acid N-hydroxysuccinimide ester and other essential bioconjugation reagents.