In the realm of biochemical and molecular biology research, accurately quantifying protein concentration is a cornerstone for reliable experimental outcomes. Among the various methods available, the Bicinchoninic Acid (BCA) assay stands out for its sensitivity, robustness, and compatibility with a wide range of biological samples. NINGBO INNO PHARMCHEM CO.,LTD. offers high-quality Bicinchoninic Acid Disodium Salt, a key reagent that empowers researchers to achieve precise protein quantification.

The BCA assay is built upon two critical reactions. Firstly, in an alkaline environment, proteins reduce Cu2+ ions to Cu1+ ions. This reduction is directly proportional to the amount of protein present in the sample. Secondly, two molecules of bicinchoninic acid chelate with each Cu1+ ion, forming a distinct purple-colored complex that exhibits strong absorbance at 562 nm. This colorimetric reaction allows for the spectrophotometric determination of protein concentration, making it an indispensable tool for researchers aiming to buy high-quality reagents. This method is particularly advantageous as it shows reduced protein-to-protein variability compared to other assays like the Bradford assay, ensuring more consistent results when you purchase chemicals from a trusted supplier.

One of the significant benefits of the BCA assay is its compatibility with detergents. Many biological samples contain detergents, which can interfere with other protein assay methods like the Lowry or Coomassie dye assays. The ability of the BCA assay to tolerate detergents (up to 5%) makes it a more versatile choice for complex sample matrices. This feature is highly valued by scientists seeking effective protein quantification methods. When seeking a reliable supplier for your research, consider NINGBO INNO PHARMCHEM CO.,LTD. for your Bicinchoninic Acid Disodium Salt needs.

Furthermore, the sensitivity of the BCA assay is noteworthy, allowing for the detection of protein concentrations as low as 0.5 μg/mL in some protocols. This makes it suitable for analyzing dilute protein samples, a common scenario in many research settings. For those looking to purchase protein determination reagents, understanding these benefits is key to selecting the best tools for their laboratory. The efficiency and reliability of this assay, powered by quality reagents from NINGBO INNO PHARMCHEM CO.,LTD., contribute significantly to the advancement of scientific discovery. If you are planning your next research project, incorporating the BCA assay with our superior grade Bicinchoninic Acid Disodium Salt is a wise choice for accurate and dependable protein quantification.