In the dynamic field of life sciences, precise control over experimental conditions is critical for achieving reproducible and meaningful results. Biological buffers play an instrumental role in this, maintaining stable pH levels that are essential for the function of enzymes, proteins, and nucleic acids. N-Cyclohexyl-3-aminopropanesulfonic acid, commonly abbreviated as CAPS, is a significant biological buffer that offers excellent performance, particularly in alkaline environments, making it a valuable reagent for a wide range of life science applications.

CAPS is a zwitterionic buffer with a pKa of approximately 10.4, rendering it effective for buffering solutions in the alkaline pH range of 9.7 to 11.1. This capability is highly sought after in various biochemical and molecular biology techniques. For instance, in the development and manufacturing of biochemical diagnostic kits, CAPS helps ensure the stability and activity of assay components, leading to more reliable diagnostic outcomes. Researchers and kit manufacturers often look to purchase CAPS from specialized suppliers who can guarantee high purity (≥99.0%) and consistent quality.

Within molecular biology, CAPS is frequently employed in DNA/RNA extraction kits and PCR diagnostic kits. The extraction of high-quality nucleic acids requires specific buffer conditions to facilitate cell lysis and nucleic acid stabilization, where CAPS can contribute to optimal performance. Similarly, in PCR, maintaining the correct pH is vital for the activity of thermostable DNA polymerases, and CAPS provides this necessary stability in the alkaline range.

Beyond molecular diagnostics, CAPS is utilized in enzyme chemistry. Many enzymes have optimal activity at alkaline pH, and CAPS is an ideal buffer to support these conditions, facilitating studies on enzyme kinetics and mechanism. Its utility also extends to analytical techniques like High-Performance Liquid Chromatography (HPLC), where it serves as a mobile phase buffer for the separation of basic compounds, contributing to resolution and peak shape.

For professionals in the life sciences industry, sourcing N-Cyclohexyl-3-aminopropanesulfonic acid involves identifying reliable manufacturers and suppliers. Many look to established chemical companies, particularly those based in China, for cost-effective procurement of high-purity reagents. When considering a purchase, buyers should prioritize suppliers who provide comprehensive product specifications and Certificates of Analysis. If your research or product development requires a robust alkaline buffer, exploring the availability and pricing of CAPS from reputable manufacturers is a wise step.