The ability to synthesize precise and high-quality oligonucleotides is fundamental to advancements in various life science fields, including diagnostics, therapeutics, and fundamental research. At the core of automated oligonucleotide synthesis are phosphoramidites, which act as activated nucleotide building blocks. For researchers focusing on RNA synthesis, N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS: 121058-88-6) is a crucial reagent. This article highlights its importance and guides on how to source it effectively.

The Chemical Architecture of CAS 121058-88-6 for RNA Synthesis

The complexity of N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a testament to the sophisticated chemistry required for efficient RNA assembly. Each component plays a vital role:

  • Cytidine Base: The core nucleobase, cytidine, is essential for RNA sequences.
  • N4-Acetyl Group: Protects the exocyclic amine of cytosine, preventing unwanted side reactions during synthesis.
  • 2'-O-TBDMS Group: The tert-butyldimethylsilyl (TBDMS) protection on the 2'-hydroxyl is critical for RNA synthesis, preventing the formation of unwanted 2'-5' linkages and ensuring the correct 3'-5' phosphodiester bond formation.
  • 5'-O-DMT Group: The dimethoxytrityl (DMT) group on the 5'-hydroxyl is a standard acid-labile protecting group, facilitating stepwise addition of nucleotides during synthesis.
  • Phosphoramidite Functionality: The cyanoethyl phosphoramidite group is the reactive moiety that undergoes coupling and subsequent oxidation to form the phosphotriester linkage.

When you aim to buy this phosphoramidite, you are acquiring a precisely engineered molecule that is central to achieving successful and reproducible RNA synthesis.

Procurement Best Practices for Phosphoramidites

For procurement managers and research scientists, selecting the right supplier for critical intermediates like CAS 121058-88-6 is a strategic decision. Here are key considerations:

  • Verify Purity and Quality: Always insist on a Certificate of Analysis (CoA) that confirms high purity (≥97%) and details analytical methods used. The supplier's adherence to stringent quality control is paramount.
  • Supplier Reputation: Partner with established manufacturers and suppliers known for their expertise in pharmaceutical intermediates and fine chemicals. A strong track record and positive customer reviews are good indicators.
  • Bulk Purchase Options and Pricing: If your needs extend beyond research quantities, explore suppliers who can offer bulk pricing and consistent availability. Many manufacturers in China are competitive in this regard.
  • Technical Support and Responsiveness: A supplier that provides responsive customer service and technical expertise can be invaluable for troubleshooting and ensuring optimal product usage.

For those seeking to purchase this vital cytidine phosphoramidite, a reputable manufacturer based in China offers a compelling combination of quality, expertise, and cost-effectiveness. By choosing a dedicated supplier, you ensure a stable supply of high-purity reagents, which is fundamental to optimizing your oligonucleotide synthesis protocols and advancing your research or product development efforts.