Understanding the Properties and Storage of Bicinchoninic Acid Disodium Salt
For any laboratory performing sensitive biochemical assays, a thorough understanding of the reagents used is critical. Bicinchoninic acid disodium salt, known by its CAS number 979-88-4, is a prime example of a chemical compound whose properties and handling directly impact experimental success. This salt plays a pivotal role in protein quantification, particularly through the BCA assay, and its careful management is essential for researchers aiming for accurate and reproducible results.
The key characteristic of Bicinchoninic acid disodium salt is its ability to react with copper(I) ions to form a stable, intensely purple-colored complex. This chromogenic property is harnessed in analytical techniques. In the BCA protein assay, proteins reduce copper(II) to copper(I) ions under alkaline conditions. Subsequently, two molecules of Bicinchoninic acid disodium salt chelate each copper(I) ion, resulting in a color change that can be measured spectrophotometrically at 562 nm. The intensity of this color is proportional to the protein concentration.
Physically, Bicinchoninic acid disodium salt typically presents as a white to light yellow powder or chunks. Its molecular formula is C20H10N2Na2O4, and it is soluble in water, which is a convenient property for preparing assay solutions. However, like many sensitive chemical compounds, it requires specific storage conditions to maintain its integrity and efficacy. Manufacturers often recommend storing it sealed in a cool (2-8°C) and dry place. This temperature range helps to prevent degradation and maintain the compound's reactivity for extended periods, typically with a self-life of one year under proper storage.
For laboratories looking to buy Bicinchoninic acid disodium salt, understanding these properties is crucial for procurement and laboratory management. Purchasing from a reliable manufacturer ensures that the product you receive meets the specified purity levels (often ≥98%), which is vital for sensitive assays. We, as a dedicated supplier, provide this essential reagent with clear guidance on its properties and optimal storage, ensuring you get the most out of your purchase.
By adhering to proper handling and storage protocols, researchers can maximize the utility of Bicinchoninic acid disodium salt, ensuring the accuracy and reliability of their protein quantification experiments. If your research requires this compound, we encourage you to buy from a trusted manufacturer that prioritizes quality and product integrity.
Perspectives & Insights
Silicon Analyst 88
“However, like many sensitive chemical compounds, it requires specific storage conditions to maintain its integrity and efficacy.”
Quantum Seeker Pro
“This temperature range helps to prevent degradation and maintain the compound's reactivity for extended periods, typically with a self-life of one year under proper storage.”
Bio Reader 7
“For laboratories looking to buy Bicinchoninic acid disodium salt, understanding these properties is crucial for procurement and laboratory management.”