Proteinase K (CAS 39450-01-6) is widely recognized for its indispensable role in DNA and RNA extraction, primarily for its ability to degrade proteins and inactivate nucleases. However, this versatile serine protease possesses a broader range of applications in research that are equally critical. As a dedicated manufacturer and supplier of high-quality biochemicals, we are keen to highlight the multifaceted utility of this enzyme.

Beyond Nucleic Acid Purification: Diverse Research Applications

While its utility in purifying genetic material is paramount, Proteinase K's robust proteolytic activity makes it valuable in several other scientific domains:

  • Protein Digestion and Analysis: In proteomics and biochemistry, Proteinase K can be used to digest specific proteins or to prepare protein samples for mass spectrometry or gel electrophoresis. Its broad specificity allows for efficient breakdown of complex protein structures.
  • Inactivation of Enzymes: Proteinase K is highly effective at inactivating a wide array of enzymes, including those that might interfere with experimental processes or downstream assays. This includes enzymes like alkaline phosphatase, which can be found in various biological preparations and assays.
  • Histone Digestion: It efficiently digests histones that are bound to genomic DNA. This is important in studies investigating chromatin structure and gene regulation, where accessing DNA requires removal of associated proteins.
  • Sample Preparation for Various Assays: In many diagnostic and research protocols, Proteinase K is employed early in sample preparation to degrade cellular components and release target molecules, ensuring cleaner and more efficient downstream processing.
  • Protein Structure Studies: Its ability to cleave peptide bonds adjacent to hydrophobic amino acids makes it a tool for probing protein structures and identifying vulnerable sites.

Ensuring Quality for Broad Applications

Regardless of the specific application, the quality of Proteinase K remains a critical factor. For researchers looking to buy Proteinase K, understanding the enzyme's purity and activity specifications is essential. As a leading manufacturer and supplier, we provide high-purity Proteinase K (99%) with guaranteed activity, suitable for all these diverse research needs. Our commitment ensures that whether you are performing standard DNA extraction or advanced protein analysis, you receive a reagent that delivers consistent and reliable performance.

Procuring Proteinase K

When seeking Proteinase K for sale, consider partnering with a supplier that can offer both quality and competitive pricing. We are dedicated to supporting scientific advancement by providing reliable biochemicals. We encourage you to explore our product offerings and request a quote for your Proteinase K needs, ensuring your research is powered by the best available reagents.