In the realm of scientific research, particularly in biochemistry and molecular biology, specialized chemical compounds are indispensable tools for unraveling complex biological pathways. 2,4-Diamino-6-hydroxypyrimidine (CAS 56-06-4) is one such compound, recognized for its specific inhibitory action on GTP cyclohydrolase I (GTPCH), a key enzyme in the de novo synthesis of tetrahydrobiopterin (BH4).

Understanding the Mechanism: GTPCH Inhibition

Tetrahydrobiopterin (BH4) is a vital cofactor involved in several critical enzymatic reactions within the human body, including the synthesis of nitric oxide (NO) and aromatic amino acids. GTPCH is the rate-limiting enzyme in the BH4 biosynthesis pathway. By selectively inhibiting GTPCH, 2,4-Diamino-6-hydroxypyrimidine effectively blocks the production of BH4. This mechanism makes it a valuable research tool for scientists studying:

  • Nitric Oxide (NO) Synthesis: BH4 is a crucial cofactor for inducible nitric oxide synthase (iNOS). Researchers utilize DAHP to investigate the role of BH4 in NO production in various cell types, such as vascular smooth muscle cells and mast cells. Understanding this interaction is key to research in cardiovascular diseases and inflammatory responses.
  • Pteridine Metabolism: The compound aids in studying the complex pathways of pteridine metabolism and the regulation of BH4 levels.
  • Enzyme Kinetics: It serves as a standard inhibitor for kinetic studies of GTPCH, helping to characterize enzyme activity and identify potential therapeutic targets.

Procurement for Research Purposes

For researchers needing to buy 2,4-Diamino-6-hydroxypyrimidine for laboratory studies, sourcing from reputable chemical suppliers is essential. Key considerations include:

  • Purity: While research-grade purity (often >98% or >99%) is required, the availability of detailed analytical data is crucial for experimental reproducibility.
  • Quantity: Researchers typically require smaller quantities compared to industrial manufacturing, so suppliers offering various pack sizes, from grams to kilograms, are ideal.
  • Availability: Ensuring a consistent supply chain means researchers can obtain the compound when needed without delays, which is critical for ongoing experiments.
  • Price: Obtaining competitive pricing, especially for academic institutions, can facilitate broader research projects.

In summary, 2,4-Diamino-6-hydroxypyrimidine is an invaluable tool for researchers investigating metabolic pathways and enzyme functions. If you are looking to buy this compound for your research, identifying a reliable supplier that offers high-purity material and responsive customer service will ensure the success of your scientific endeavors. We are a dedicated supplier capable of meeting your research needs for this specific chemical intermediate.