D-Luciferin, chemically known as (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, is the key organic molecule responsible for light emission in firefly bioluminescence. Its unique chemical structure and properties make it an exceptional substrate for the luciferase enzyme, forming the basis for numerous scientific research applications.

Understanding the chemical and physical properties of D-Luciferin is crucial for its effective use in the laboratory. Typically supplied as a powder, its appearance ranges from white to faint yellow. Its molecular weight is approximately 280.32 g/mol. For optimal performance in bioluminescent assays, a high purity level, often exceeding 98% as confirmed by HPLC, is essential. This high purity ensures that the light emitted is directly proportional to the amount of luciferase or the biological activity being measured, minimizing experimental variability.

Solubility is another critical factor. D-Luciferin exhibits good solubility in various solvents, which facilitates its preparation into stock solutions for experimental use. This characteristic allows for flexible application in both in vitro and in vivo studies. For most research applications, D-Luciferin is prepared in aqueous buffers or cell culture media. For researchers looking to buy D-Luciferin, understanding its solubility profile helps in designing effective experimental protocols.

Proper storage is vital for maintaining the integrity and efficacy of D-Luciferin. It is typically recommended to store D-Luciferin at -20°C, protected from light and moisture. When stored under these conditions, it generally maintains its stability for up to two years. As a responsible D-Luciferin manufacturer and supplier, we ensure our products are packaged appropriately, often in vacuum-sealed foil bags, to preserve their quality during transit and storage.

The performance of D-Luciferin as a bioluminescent substrate is well-established. Its efficient interaction with luciferase to produce a strong light signal makes it ideal for a wide range of applications, from basic molecular biology research to advanced in vivo imaging. When sourcing D-Luciferin, partnering with a manufacturer that emphasizes quality control and provides comprehensive technical data ensures you receive a product that consistently delivers reliable results for your scientific endeavors.